Related Experiment Video
Updated: May 1, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases
Qingshang Bi1, Mengru Liu2,3, Li Yan4
1Department of Clinical Laboratory, Third Sanatorium, Air Force Healthcare Center for Special Services, Hangzhou, China.
Abstract:
Rapid detection of infectious diseases is critical for global public health prevention and control. However, the use of traditional molecular diagnostic methods, including PCR, has been limited because of their cumbersome procedures, complex equipment requirements, operation at different temperatures, and the level of expertise required for operation. Isothermal amplification technology (IAT) provides a rapid, sensitive, specific, simple and less costly method for diagnosing infectious diseases, which has led to revolutionary breakthroughs in molecular diagnostics. This paper summarizes recent progress in IAT technology, which focuses on the principles and applications of core technologies such as NASBA, LAMP, RPA, and RAA. In addition, the combination of IATs with the CRISPR/Cas system, which further revolutionizes nucleic acid detection technology, is explored in this review.
More Related Videos
09:36Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Published on: February 3, 2021
06:11Author Spotlight: Advancing Rapid Detection of Respiratory Pathogens Using Microfluidic Chip
Published on: March 29, 2024
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Applications of Molecular Taxonomy
Automated Microbial Diagnostics