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Related Concept Videos

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
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Related Experiment Video

Updated: Sep 14, 2025

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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CacyBP/SIP - RPL6 interaction: potential influence on ribosome function.

Ewelina Jurewicz1, Małgorzata Maksymowicz-Trivedi1, Omid Saberi-Khomami1

  • 1Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, Warsaw, 02-093, Poland.

Amino Acids
|July 21, 2025
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Summary
This summary is machine-generated.

CacyBP/SIP directly interacts with ribosomal protein RPL6, impacting ribosome function and protein synthesis. Silencing CacyBP/SIP reduced nascent polypeptide synthesis and altered stress response in neuroblastoma cells.

Keywords:
CacyBP/SIPMolecular modelingProtein synthesisRPL6Ribosome

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • CacyBP/SIP (also known as CacyBP) has a previously identified interaction with NPM1, a key player in ribosome biogenesis.
  • This study investigates the role of CacyBP/SIP in the broader context of ribosome biogenesis and function.

Purpose of the Study:

  • To determine the impact of CacyBP/SIP on ribosome biogenesis and function.
  • To identify potential ribosomal protein targets of CacyBP/SIP.
  • To elucidate the functional consequences of CacyBP/SIP-ribosome interactions on protein synthesis.

Main Methods:

  • Mass spectrometry to identify CacyBP/SIP interacting partners.
  • Biochemical assays to confirm direct interaction between CacyBP/SIP and RPL6.
  • In silico analysis to map protein-binding domains.
  • O-propargyl-puromycin (OPP) labeling to assess nascent polypeptide synthesis.
  • Heat shock experiments to evaluate stress-induced protein production (Hsp70) in CacyBP/SIP-silenced cells.

Main Results:

  • Mass spectrometry identified several ribosomal proteins (RPs) as potential CacyBP/SIP targets, with RPL6 showing high-quality scores.
  • Biochemical and in silico methods confirmed a direct interaction between CacyBP/SIP and RPL6, identifying involved protein domains.
  • CacyBP/SIP silencing in neuroblastoma cells led to a significant decrease in cells exhibiting perinuclear staining for nascent polypeptides.
  • CacyBP/SIP-silenced cells showed markedly higher Hsp70 production under heat shock compared to control cells, suggesting impaired protein synthesis regulation.

Conclusions:

  • CacyBP/SIP directly interacts with RPL6 and potentially other RPs.
  • CacyBP/SIP appears to play a role in ribosome function and the efficiency of protein synthesis.
  • The findings suggest CacyBP/SIP's involvement in cellular protein synthesis regulation, possibly via its interaction with ribosomal components.