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Updated: Sep 14, 2025

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

Transcriptome profiling and co-expression network analysis of 96 Haematococcus pluvialis samples.

Bin Hu¹, Kai Liu¹, Feichao Du¹

¹Key Laboratory of Aquacultural Biotechnology of Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, 315832, China.

Scientific Data

|July 21, 2025

Summary

This summary is machine-generated.

This study used RNA-seq to analyze Haematococcus pluvialis, revealing gene networks involved in astaxanthin production and cell wall formation. The findings offer insights into optimizing microalgal biomass and valuable compounds.

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Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

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Published on: February 18, 2009

Area of Science:

Microalgal biotechnology
Transcriptomics
Carotenoid biosynthesis

Background:

Haematococcus pluvialis is a key microalga for astaxanthin production.
Improving astaxanthin yield requires understanding growth, biomass optimization, and cell wall development.

Purpose of the Study:

To generate and analyze a comprehensive transcriptomic dataset for H. pluvialis.
To identify gene co-expression networks regulating key physiological processes.
To functionally annotate previously uncharacterized genes.

Main Methods:

RNA sequencing (RNA-seq) of 96 H. pluvialis samples.
Gene expression quantification (TPM) and co-expression network construction.
Functional classification and pathway analysis of gene modules.

Main Results:

Generated 96.7 GB of high-quality RNA-seq data.
Identified 39 co-expression modules, revealing gene relationships.
Provided functional annotations for 8,598 uncharacterized genes involved in metabolism and genetic processing.

Conclusions:

The dataset provides a valuable resource for understanding H. pluvialis molecular biology.
Insights gained can guide strategies for enhanced astaxanthin production and biomass.
Facilitates research into growth regulation and secondary cell wall synthesis.