SPC25 as a prognostic and immune-related biomarker in lung adenocarcinoma

In the development of human cancers, alterations in the process of cell mitosis frequently play a role. In recent years, an increasing number of researchers have focused on the phenomenon of cancer resulting from kinetochore-microtubule attachment imbalance.Spindle pole body component 25 (SPC25) is essential for the organization of the spindle devices and chromosome separation. SPC25 has a significant role in the occurrence and development of malignant tumors, but its role in lung adenocarcinoma has not been established. This study utilizes the TCGA database and centrosome assembly checkpoint specialized resources to explore the molecular mechanisms of lung adenocarcinoma and its interaction with the immune system. By using the "limma" and "survival" R software packages and Venn diagram analysis, we identify differentially expressed mRNAs that are associated with lung adenocarcinoma prognosis and play a role in centrosome assembly checkpoint regulation, and determine SPC25 as the target gene. We use the "pROC" software package to draw ROC curves to evaluate its diagnostic potential, perform chi-square tests and logistic regression models to analyze its relationship with clinical pathological features. Cox regression analysis deepens our understanding of its prognostic evaluation role, and enrichment analysis reveals its biological functions and signaling pathways. We also explore the correlation between SPC25 and lung adenocarcinoma immune infiltration by combining ssGSEA and Spearman analysis. The objective of this study was to investigate the association between SPC25 and the prognosis as well as immune infiltration in lung adenocarcinoma, aiming to establish a crucial molecular foundation for early non-invasive diagnosis and immunotherapy of lung adenocarcinoma.