Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Nucleus Transcriptomics of the Mouse Medial Preoptic Area Reveals Sex-Dependent Molecular Signatures of Social Dominance.

bioRxiv : the preprint server for biology·2026
Same author

The role of ovarian hormones in risk aversion in female rats.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Sex- and Lineage-Dependent Transgenerational Effects of Vinclozolin and Flutamide on Rat Development and Behavior.

Journal of the Endocrine Society·2025
Same author

Sex differences in microglia morphology and function across the lifespan are mediated by the early hormone environment.

Brain, behavior, and immunity·2025
Same author

Rapid Changes in Cholinergic Signaling, Myelination, and Thyroid Signaling Pathway Gene Expression in Amygdala Subnuclei in Response to Social Status Maintenance and Reorganization.

The European journal of neuroscience·2025
Same author

Transcriptomic Changes Across the HPG Axis Following Prenatal Exposure to the EDC Mixture NeuroMix.

Endocrinology·2025
Same journal

Blood-Based MicroRNAs as Biomarkers of Insulin Resistance in Childhood Obesity: A Systematic Review and meta-analysis.

Molecular and cellular endocrinology·2026
Same journal

Female-specific metabolic genetic liability reveals a separable metabolic dimension of polycystic ovary syndrome.

Molecular and cellular endocrinology·2026
Same journal

The kisspeptin analog C6 elicits greater tachyphylaxis and transcriptional activation than kisspeptin-10 and -54.

Molecular and cellular endocrinology·2026
Same journal

G1 regulation of BK<sub>Ca</sub> channel leads to decreased migration of senescent pericytes and improved age-related hearing loss.

Molecular and cellular endocrinology·2026
Same journal

Maternal butyrate administration ameliorates fetal fatty liver and maternal metabolic alterations related to maternal obesity.

Molecular and cellular endocrinology·2026
Same journal

Retraction notice to " Steroid-induced oocyte maturation in Indian shad Tenualosa ilisha (Hamilton, 1822) is dependent on phosphatidylinositol 3 kinase but not MAP kinase activation" [Mol. Endocrinol. 390 (2014) 26-33].

Molecular and cellular endocrinology·2026
See all related articles

Related Experiment Video

Updated: Sep 14, 2025

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
11:48

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Published on: July 24, 2013

16.0K

Transcriptomic profiling of hypothalamic development in female rats.

Emily N Hilz1, Madeline Streifer1, Tyler M Milewski1

  • 1Division of Pharmacology & Toxicology, The University of Texas at Austin, Austin, TX, 78712, USA; Center for Molecular Carcinogenesis & Toxicology, The University of Texas at Austin, Austin, TX, 78712, USA.

Molecular and Cellular Endocrinology
|July 22, 2025
PubMed
Summary
This summary is machine-generated.

Hypothalamic development in female rats shows significant gene expression changes from infancy to puberty in both the arcuate nucleus (ARC) and anteroventral periventricular nucleus (AVPV), with FSH correlating strongly with gene activity.

Keywords:
Anteroventral periventricular nucleus (AVPV)Arcuate nucleus (ARC)Energy balanceHypothalamusReproductionTranscriptomics

More Related Videos

Author Spotlight: Hypothalamic Neural Mechanism Insights
09:29

Author Spotlight: Hypothalamic Neural Mechanism Insights

Published on: August 4, 2023

3.9K
Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
09:12

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Published on: November 14, 2018

8.0K

Related Experiment Videos

Last Updated: Sep 14, 2025

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
11:48

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Published on: July 24, 2013

16.0K
Author Spotlight: Hypothalamic Neural Mechanism Insights
09:29

Author Spotlight: Hypothalamic Neural Mechanism Insights

Published on: August 4, 2023

3.9K
Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
09:12

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Published on: November 14, 2018

8.0K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Endocrinology

Background:

  • The hypothalamic arcuate nucleus (ARC) and anteroventral periventricular nucleus (AVPV) are crucial for regulating reproduction, energy balance, stress, and neuromodulation.
  • These regions undergo significant developmental changes in neural and glial populations, essential for adult functions.

Purpose of the Study:

  • To comprehensively compare unbiased transcriptional profiles across key developmental stages (infantile, peripubertal, adult) in the ARC and AVPV of female rats.
  • To identify developmental gene expression patterns and their correlation with physiological hormonal changes.

Main Methods:

  • Utilized 3' targeted RNA sequencing to profile gene expression in the ARC and AVPV at postnatal days 8, 30, and 60.
  • Performed unbiased and a priori differential gene expression analyses, focusing on genes related to reproduction, metabolism, stress, and neuromodulation.
  • Measured serum hormone levels as a physiological correlate.

Main Results:

  • Robust differential gene expression was observed between infantile and prepubertal stages in both ARC and AVPV, with considerable transcriptional overlap.
  • Fewer, more region-specific transcriptional changes occurred during the transition to adulthood.
  • Gene ontology analysis indicated coordinated developmental programming before puberty, including downregulation of developmental processes and upregulation of metabolic/regulatory pathways.
  • The AVPV exhibited continued transcriptional remodeling in adulthood, while the ARC remained relatively stable.
  • Follicle-stimulating hormone (FSH) showed the strongest correlation with a priori selected hypothalamic gene expression.

Conclusions:

  • Hypothalamic development involves significant transcriptional shifts, particularly before puberty, with distinct adult remodeling patterns in ARC and AVPV.
  • These findings provide a valuable reference for understanding hypothalamic development and hormone-gene interactions throughout life stages in female rats.