Related Experiment Video
Updated: Sep 14, 2025
Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
Published on: June 2, 2015
Dietary Fat Intake Attenuates Vitamin A Deficiency-Associated Elastic Fiber Remodeling and Lipid Reduction in the
Lisa-Marie Hoy1, Tabea Meier1, Natascha Mierswa1
1Hannover Medical School, Institute of Functional and Applied Anatomy, Hannover, Germany.
Background:
Vitamin A deficiency (VAD) and obesity are widespread nutrition-related health conditions that are independently associated with pulmonary remodeling processes linked to lung function decline and respiratory diseases.
Objectives:
This study tested the hypothesis that VAD-related pulmonary alterations are aggravated by diet-induced obesity.
Methods:
Eight-week-old C57BL/6J/129Sv mice with a deletion of lecithin-retinol-acyltransferase (Lrat-; impaired vitamin A storage) were fed vitamin A deficient control diet (CD, n = 13) or high-fat diet (HFD, n = 15) to induce VAD in lean (CD-VAD, n = 13) or obese (HFD-VAD, n = 13) mice. Wild-type mice receiving vitamin A-containing CD or HFD served as controls. After 20 wk, lungs were subjected to structural and molecular analyses by stereology, western blot, and high-pressure liquid chromatography-mass spectrometry. Statistics used were 2-way analysis of variance.
Results:
Pulmonary vitamin A reserves were efficiently depleted in CD-VAD and HFD-VAD (P < 0.001 compared with controls). In CD-VAD, 76% of pulmonary elastic fibers appeared densely packed (CD: 53%, P < 0.01), and expression of fibrillin was 110% higher compared with CD (P < 0.01), indicating a higher septal microfibril content. Elastin expression was slightly reduced in HFD-groups (HFD: 14%, HFD-VAD: 16% of respective controls, both P < 0.05), whereas neither diet nor VAD affected expression levels of collagen I or III. Lipid droplet volumes decreased by 32% in septal fibroblasts (P < 0.05) and by 53% in alveolar epithelial type 2 (AE2) cells in CD-VAD, compared with CD. HFD alone led to a 20% reduction in lung airspace volume, a 13% decrease in septal surface area, and a 15% reduction in AE2 cell numbers compared with CD. These VAD- and obesity-related changes were alleviated or absent in HFD-VAD.
Conclusions:
VAD-induced elastic fiber remodeling and lipid droplet reduction in the alveolar region of lean mice, whereas HFD resulted in smaller lungs containing less AE2 cells. Both VAD- and obesity-related effects were attenuated in HFD-VAD, indicating mutually mitigating effects.
