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Related Experiment Video

Updated: Sep 14, 2025

Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
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Minimally Invasive Chemical Biopsy Needle with Self-Wettable Extraction Phase For In Vivo Tissue Sampling During

Runshan Will Jiang1, Wei Zhou1, Marcelo Cypel2

  • 1Department of Chemistry, University of Waterloo, Waterloo, ON, N2L 3G1, Canada.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|July 22, 2025
PubMed
Summary

This study introduces a less invasive chemical biopsy technique using a novel solid-phase microextraction (SPME) device. This innovation enables real-time in vivo monitoring of drug concentrations during surgery without solvents.

Keywords:
drug monitoringin vivo lung perfusionin vivo samplingsolid‐phase microextractiontissue sampling

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Pharmacology

Background:

  • Conventional tissue biopsy is invasive and can be challenging for continuous monitoring.
  • Solid-phase microextraction (SPME) offers a less invasive alternative for sampling biological analytes.
  • There is a need for real-time monitoring of therapeutic agents in vivo.

Purpose of the Study:

  • To develop and validate a novel, self-protective SPME device for in vivo chemical biopsy.
  • To enable solvent-free extraction and analysis of endogenous and exogenous substances in biological tissues.
  • To demonstrate the feasibility of real-time drug monitoring during medical procedures.

Main Methods:

  • A biocompatible, self-protective acupuncture needle coated with naturally wettable sorbent particles was designed.
  • The SPME device was used for in vivo sampling of anti-cancer drugs in animal models and human patients.
  • Liquid chromatography-mass spectrometry (LC/MS) was employed for drug concentration determination.
  • A microfluidic open interface (MOI) was proposed for direct analyte desorption and MS detection.

Main Results:

  • The developed SPME device provided effective, solvent-free analyte extraction.
  • Successful sampling and quantification of anti-cancer drugs were achieved in vivo during lung perfusion surgery.
  • The proof-of-concept MOI demonstrated potential for on-site, real-time drug monitoring.

Conclusions:

  • The novel SPME device offers a significantly less invasive approach for chemical biopsy and in vivo monitoring.
  • This technology facilitates real-time assessment of drug concentrations, potentially improving therapeutic outcomes.
  • Future applications include on-site, real-time drug monitoring during surgeries and other medical interventions.