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Metalens-Based Dual Light-Sheet Fluorescence Microscopy.

Yuan Luo1,2,3,4, Chun-Chun Chang1,5, Hung-Chuan Hsu1,5

  • 1Institute of Medical Device and Imaging, National Taiwan University, Taipei, 10051, Taiwan.

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|July 22, 2025
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Summary
This summary is machine-generated.

This study introduces a metalens-based dual-sided illumination light-sheet fluorescence microscopy (LSFM) to overcome traditional LSFM

Keywords:
dual‐sided illuminationfluorescence imageslight‐sheet fluorescence microscopymetalensmice lung tissues

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Area of Science:

  • Biomedical Optics
  • Microscopy Technology
  • Advanced Imaging Techniques

Background:

  • Traditional light-sheet fluorescence microscopy (LSFM) suffers from stripe and shadow artifacts due to single-sided illumination, leading to uneven contrast and reduced image uniformity, especially in non-transparent samples.
  • These artifacts compromise the quality and reliability of volumetric imaging, limiting the diagnostic potential of LSFM in biological and medical applications.

Purpose of the Study:

  • To develop a compact and efficient LSFM system that eliminates shadow artifacts and improves image uniformity for enhanced in situ tissue imaging.
  • To leverage metalens technology for dual-sided illumination, enabling uniform sample illumination and high-resolution imaging.

Main Methods:

  • Integration of a pair of cylindrical metalenses into an LSFM system to generate twin, identical light-sheets from opposite sides of the sample.
  • System-level structure optimization of the metalens-based dual-sided illumination LSFM (MDI-LSFM) through rigorous experimental setup.
  • Demonstration of the system's capability using ex vivo images of mice lung tissues.

Main Results:

  • The developed MDI-LSFM system successfully eliminated shadow artifacts and achieved uniform sample illumination.
  • Achieved a lateral resolution of 1.7 µm and an optical sectioning capability of 6.8 µm.
  • Demonstrated a large field of view, high resolution, and fast imaging, surpassing traditional LSFM limitations.

Conclusions:

  • Metalens-based dual-sided illumination LSFM offers a compact, efficient, and artifact-free solution for high-quality volumetric imaging.
  • The MDI-LSFM system significantly enhances in situ tissue imaging and diagnostics, paving the way for advanced biomedical applications.
  • This innovative approach simplifies system configuration while delivering superior imaging performance compared to conventional LSFM techniques.