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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Homologous Recombination02:31

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Synthetic Biology02:55

Synthetic Biology

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Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Updated: Sep 14, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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Biosafety considerations triggered by genome-editing technologies.

Xinxin Li1, Yuanjiao Gao1, Ziyu Zhang1

  • 1Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.

Biosafety and Health
|July 22, 2025
PubMed
Summary

Gene editing technologies like CRISPR-Cas9 offer medical advancements but pose biosafety risks. Ensuring safe application requires addressing off-target effects, genomic instability, and ethical concerns through policy and regulation.

Keywords:
BiosafetyClustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9)Ethical considerationsGenome-editing technologiesRegulatory framework

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Area of Science:

  • Biotechnology
  • Genetics
  • Medical Research

Background:

  • Deoxyribonucleic acid (DNA) structure discovery spurred gene editing advancements.
  • Genome-editing technologies, particularly CRISPR-Cas9, present new biomedical opportunities.

Purpose of the Study:

  • To review the current status of gene editing in medicine.
  • To identify and discuss potential biosafety risks associated with gene editing.
  • To explore strategies for ensuring the safe and responsible application of gene editing technologies.

Main Methods:

  • Literature review of gene editing technologies and their applications.
  • Analysis of potential risks including off-target effects and genomic instability.
  • Discussion of policy, regulatory, and technical measures for biosafety.

Main Results:

  • Gene editing offers significant potential for biomedical research and clinical applications.
  • Biosafety concerns, including off-target effects and ethical issues, require careful consideration.
  • Clinical safety and efficacy require further verification.

Conclusions:

  • Responsible implementation of gene editing necessitates addressing safety, ethical, and legal dimensions.
  • Policies, regulations, and technical advancements are crucial for mitigating risks.
  • A comprehensive perspective is needed for the safe advancement of gene editing in medicine.