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Updated: Sep 14, 2025
Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications
Published on: April 30, 2014
Comparison of cellular activity and gene expression in repaired versus reconstructed anterior cruciate ligaments: A
Edoardo Monaco1, Alessandro Annibaldi1, Adnan Saithna2
1Department of Orthopaedics Sant'Andrea Hospital, La Sapienza University of Rome Rome Italy.
Purpose:
The aim of this study was to compare gene expression and cell biology in biopsy specimens from repaired and reconstructed anterior cruciate ligaments.
Methods:
A biopsy of the repaired and reconstructed anterior cruciate ligaments was performed. Gene expression analysis of alpha smooth muscle alpha actin via immunofluorescence and gene expression analysis of Collagen I, Collagen III, Collagen I-III ratio, and p16 via reverse transcription polymerase chain reaction was performed. In addition, cell motility and migration were quantitatively assessed. Data analysis was performed using analysis of variance (ANOVA) and Tukey's multiple comparison tests.
Results:
Twenty-five biopsies, 15 from repaired anterior cruciate ligaments and 10 from reconstructed anterior cruciate ligaments were obtained. Gene expression analysis showed a higher expression of Collagen I in the reconstruction group (p = 0.0027) while the expression of alpha smooth muscle actine, was significantly greater for the repair group (p = 0.001). Quantitative analysis demonstrated significantly higher cell motility in the cells from repaired ligament compared to cells cultured from grafts (43 vs. 54% residual open area from scratch, p = 0.0001 at 24 h; 10 vs. 17% residual open area from scratch, p = 0.001 at 48 h).
Conclusions:
Molecular analysis of repaired and reconstructed anterior cruciate ligaments demonstrates significant differences with respect to collagen quality, α-SMA, and p16. dPCs cultured from biopsies tissue showed a greater cellular activation and migration capacity in the repaired ligament. The clinical importance of these findings is a confirmation that repaired anterior cruciate ligament tissue exhibits a cellular behaviour consistent with excellent biological healing potential.
Level Of Evidence:
Level III, case-control study.
