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Recovery of phenotypically sorted cells using droplet-digital microfluidics.

Zhiyang Deng1,2, James M Perry2, Marian Weiss3

  • 1Department of Electrical and Computer Engineering, Concordia University, Montréal, Canada. steve.shih@concordia.ca.

Lab on a Chip
|July 22, 2025
PubMed
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This study introduces an integrated microfluidic platform to improve the recovery of single cells from droplets. This new method significantly enhances cell recovery rates for applications like antibody discovery.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Droplet microfluidics is vital for high-throughput single-cell analysis and antibody discovery.
  • Current cell recovery rates from sorted droplets are inefficient, limiting applications.
  • Rare cell isolation remains a significant challenge in biological screening.

Purpose of the Study:

  • To develop an integrated droplet-digital microfluidic platform for enhanced isolation and recovery of single cells.
  • To improve the recovery rate of individual cells and beads from droplets.
  • To apply the developed system to antibody discovery workflows.

Main Methods:

  • Integration of droplet generation with digital microfluidics for precise cell handling.
  • Development of a novel platform for on-demand control of droplet sorting and cell recovery.

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  • Application of the system for isolating rare single cells in antibody discovery.

    • Main Results:

    • Achieved an 18-fold increase in the recovery rate of individual cells and beads from droplets.
    • Demonstrated successful application of the platform for antibody discovery.
    • Overcame limitations of conventional droplet sorting methods.

    Conclusions:

    • The integrated droplet-digital microfluidic platform significantly improves single-cell recovery rates.
    • This technology has broad implications for advancing single-cell -omics and antibody discovery.
    • The combination of high-throughput droplet generation and digital microfluidics offers a powerful tool for biological research.