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Related Experiment Video

Updated: Sep 14, 2025

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Poplar Adventitious Roots Induced by Stem Canker Pathogens: An Experimental System for Studying Roots Biology and Light Response-Related Processes

Published on: October 11, 2024

PagHB7/PagABF4-PagEPFL9 Module Regulates Stomatal Density and Drought Tolerance in Poplar.

Yufei Xia^1,2, Ruihua Guo^1,2, Te Lu^1,2

¹State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.

Plant Biotechnology Journal

|July 22, 2025

Summary

Homeobox 7 (HB7) and ABRE binding factor 4 (ABF4) regulate poplar stomatal density and drought tolerance by targeting Epidermal patterning factor-like 9 (EPFL9). This reveals new genetic resources for improving plant growth and stress resistance.

Keywords:

PagABF4 PagHB7 PagEPFL9 drought tolerance poplar stomatal density

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Published on: February 9, 2024

Area of Science:

Plant Biology
Molecular Genetics
Plant Physiology

Background:

Epidermal patterning factor-like 9 (EPFL9) is known to influence stomatal development in poplar.
The roles of homeobox 7 (HB7) and ABRE binding factor 4 (ABF4) in regulating stomatal density and drought tolerance via EPFL9 in poplar remain uncharacterized.

Purpose of the Study:

To investigate the regulatory network involving PagEPFL9, PagHB7, and PagABF4 in poplar.
To elucidate the mechanism by which these genes control stomatal development and drought tolerance.

Main Methods:

CRISPR/Cas9 gene editing to create PagEPFL9 mutants and overexpression lines.
Yeast one-hybrid, yeast two-hybrid, bimolecular fluorescence complementation, and dual luciferase reporter assays to study gene interactions.
Electrophoretic mobility shift assays and GST pull-down assays to confirm protein-DNA and protein-protein interactions.

Main Results:

PagEPFL9 is localized in guard cells and responds to drought stress. Loss of function in PagEPFL9 reduced stomatal density, inhibited growth, and enhanced drought resistance.
PagHB7 acts as an upstream regulator of PagEPFL9. PagHB7 interacts with PagABF4, and PagABF4 enhances PagHB7's inhibitory effect on PagEPFL9.
Knockout of PagHB7 increased stomatal density and reduced drought tolerance. Overexpression of PagABF4 decreased stomatal density and potentially increased drought tolerance.

Conclusions:

PagHB7 and PagABF4 form an interacting complex that regulates stomatal density by targeting PagEPFL9 in poplar.
This regulatory pathway significantly impacts plant growth and drought resistance, offering valuable insights for crop improvement.