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A novel device for buffy coat collection.

Kyle Christian Hardy1, Lisa Arrigo1, Jennifer Campbell1

  • 1Rudy L. Ruggles Biomedical Research Institute, Nuvance Health, Danbury, CT, USA.

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Summary

A new multichannel aspiration device efficiently collects buffy coat from blood, crucial for rare cell detection like circulating tumor cells (CTCs). This low-cost technology improves cell recovery and preserves morphology for diagnostics.

Keywords:
Buffy coat collectionaspiration devicecell recovery efficiencycirculating tumor cellsliquid biopsy

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Area of Science:

  • Biomedical Engineering
  • Cellular Biology
  • Diagnostic Technologies

Background:

  • Buffy coat collection is vital for isolating rare circulating cells, including circulating tumor cells (CTCs).
  • Conventional buffy coat methods suffer from low yields, erythrocyte contamination, and high costs, hindering clinical utility.
  • Improved methods are needed for efficient and cost-effective buffy coat collection.

Purpose of the Study:

  • To develop and evaluate a novel multichannel aspiration device for buffy coat collection.
  • To assess the device's efficiency in recovering rare cells, modeled as spiked-in myeloma cells.
  • To determine the device's performance across various concentrations of target cells.

Main Methods:

  • Development of a novel multichannel aspiration device for buffy coat isolation.
  • Use of spiked-in myeloma cells to simulate circulating tumor cells (CTCs) at different concentrations (12, 30, 300 cells/mL).
  • Evaluation of cell recovery rates and assessment of cell morphology preservation using immunofluorescent imaging.

Main Results:

  • The multichannel aspiration device achieved high recovery rates for spiked-in cells, up to 98% at high concentrations and 89% at low concentrations.
  • Minimal erythrocyte contamination was observed with the novel device.
  • Immunofluorescent imaging confirmed that cell morphology was preserved during the collection process.

Conclusions:

  • The novel multichannel aspiration device offers an efficient and effective method for buffy coat collection.
  • This technology provides a low-cost alternative with high cell recovery and preserved morphology.
  • The device has significant potential for broad application in clinical diagnostics and research, particularly for rare cell detection.