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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Sep 14, 2025

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Examination of Thymic Positive and Negative Selection by Flow Cytometry

Published on: October 8, 2012

OMIP-116: A 39-Color Full Spectrum Flow Cytometric Panel to Deeply Characterize Human Thymopoiesis.

Simone Balin^1,2, Paolo Marzano^1,2, Daniele Manganaro³

¹Unit of Clinical and Experimental Immunology, Department of Medical Biotechnologies and Translational Medicine, University of Milan, Segrate, Milan, Italy.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|July 22, 2025

Summary

This summary is machine-generated.

We developed a 39-color flow cytometry panel to analyze T cell development in the thymus. This advanced tool aids in understanding immune disorders and profiling peripheral T cells, including unconventional subsets.

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Last Updated: Sep 14, 2025

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Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

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Flow Cytometric Characterization of Murine B Cell Development

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

T cell development in the thymus is crucial for adaptive immunity.
Understanding thymopoiesis is key to diagnosing and treating immune dysregulation.
Current methods may lack the resolution to fully characterize T cell maturation stages.

Purpose of the Study:

To develop and validate a high-dimensional flow cytometry panel for deep characterization of human T cell development.
To enable detailed analysis of intrathymic T cell maturation and identify deviations from normal thymopoiesis.
To provide a versatile tool for studying immune deficiencies, thymic lesions, and immunosenescence.

Main Methods:

Design and optimization of a 39-color (43-parameter) full spectrum flow cytometry panel.
Utilizing best practices for multiparametric flow cytometry.
Validation using appropriate negative and positive controls.

Main Results:

The developed panel allows high-resolution characterization of intrathymic T cell development.
It enables the definition of T cell maturation stages and identification of aberrant thymopoiesis.
The panel is also applicable for comprehensive profiling of peripheral T cells, including unconventional subsets.

Conclusions:

This 39-color flow cytometry panel is a valuable tool for studying T cell development and immune dysregulation.
It offers unprecedented resolution for analyzing thymopoiesis and peripheral T cell populations.
The panel facilitates research into conditions like immune deficiencies, thymic lesions, immunosenescence, and the role of unconventional T cells.