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Updated: Sep 14, 2025
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Published on: June 8, 2020
Identification of potential targets in bone destruction by Talaromyces marneffei: Insights from data-independent
Junhong Zhou1, Deshuang Xi1, Yilin Teng1
1Department of Spine and Osteopathy Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530000, China.
Abstract:
To investigate the molecular mechanisms of Talaromyces marneffei (TM)-induced bone destruction through proteomic analysis using Data-Independent Acquisition (DIA) technology. Bone tissue samples were collected from eight patients (four TM-infected cases, four non-infectious controls). Samples underwent histopathological evaluation (Hematoxylin and Eosin Staining and Wright-Giemsa staining), DIA proteomics analysis, and protein validation through immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). Comparative analysis between Control (Con) and Infected (Inf) groups showed similar demographics but significantly elevated inflammatory markers in Inf. Histopathology revealed extensive bone destruction, marked inflammatory infiltration, fibrinoid necrosis, and altered hematopoietic cell populations in Inf specimens compared to Con. DIA proteomics identified 5930 quantifiable proteins, with 509 differentially expressed proteins (DEPs) between groups. Gene ontology and Kyoto encyclopedia of genes and genomes pathway analyses revealed significant enrichment of inflammation and immune response-related functions in Inf. COMMD1 was significantly downregulated while IL-17 was upregulated in Inf, as validated by immunohistochemistry and ELISA. DIA proteomics identified downregulated COMMD1 and upregulated IL-17 in TM-induced bone destruction, suggesting potential diagnostic biomarkers and therapeutic targets through inflammatory pathway modulation.
Insights
Talaromyces marneffei infection causes significant bone destruction by altering immune responses. Proteomic analysis identified COMMD1 downregulation and IL-17 upregulation, suggesting new diagnostic and therapeutic targets for this fungal infection.
Area of Science:
- Mycology
- Immunology
- Proteomics
Background:
- Talaromyces marneffei (TM) infection is a serious opportunistic fungal infection.
- Bone destruction is a severe complication of TM infection, but its molecular mechanisms remain unclear.
Purpose of the Study:
- To elucidate the molecular mechanisms underlying TM-induced bone destruction.
- To identify potential biomarkers and therapeutic targets using proteomic analysis.
Main Methods:
- Bone tissue samples from TM-infected patients and controls were analyzed using Data-Independent Acquisition (DIA) proteomics.
- Histopathology, immunohistochemistry, and ELISA were employed for validation.
Main Results:
- DIA proteomics identified 509 differentially expressed proteins (DEPs) between infected and control groups.
- Gene ontology and pathway analyses highlighted enrichment of inflammation and immune response pathways.
- COMMD1 was significantly downregulated, and IL-17 was upregulated in TM-infected bone tissues.
Conclusions:
- TM infection induces significant bone destruction via modulation of inflammatory and immune pathways.
- Downregulated COMMD1 and upregulated IL-17 are potential biomarkers for TM-induced bone destruction.
- Targeting these pathways may offer therapeutic strategies for managing TM bone disease.
