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Published on: December 21, 2014
Development of Low-Immunogenic AFPs for Cell Cryopreservation by Hydrophilic Modification
Feng Shen1,2, Xiaoying Jiang1,2, Andrew B Zhu3
1Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China.
None:
The successful application of antifreeze proteins (AFPs) for cell cryopreservation is limited by their immunogenicity. In this work, a strategy has been reported to reduce the immunogenicity of AFPs by hydrophilic modification. Two hydrophilic polypeptides (KE and GES) were designed and conjugated to natural Marinomonas primoryensis AFPs (MpAFPs) to prepare AKE and AGES. In vivo tests showed that AKE and AGES exhibited 48% and 42% lower immunogenicity than natural MpAFP, respectively. Biocompatibility evaluation using a mouse model also demonstrated the proteins' negligible adverse effects on hepatic function, myocardial enzymes, and renal function. AKE and AGES were successfully used in the cryopreservation of NIH/3T3 cells and achieved survival rates of 96% and 87%, respectively. Taken together, our results lay the foundation for the development of next-generation low-immunogenic AFPs for cell and tissue cryopreservation.
