Electrochemical PSA Detection Performance of an Innovative Immunosensor

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Analytical Chemistry

Background:

• Prostate-specific antigen (PSA) is a key biomarker for prostate cancer detection and monitoring.
• Current PSA detection methods like ELISA are costly and time-consuming.
• Biosensing offers a promising alternative for rapid and sensitive PSA analysis.

Purpose of the Study:

• To develop a novel electrochemical immunosensor for sensitive and selective PSA detection.
• To synthesize and characterize key nanomaterials for immunosensor fabrication.
• To evaluate the performance of the developed immunosensor for clinical applications.

Main Methods:

• Synthesis of thiophene appended cobalt(II) phthalocyanine (CoPc), its polymer (Poly-Pc), and CuCoFe2O4@MoS2-NH2 nanomaterials.
• Immobilization of anti-PSA antibodies onto the CuCoFe2O4@MoS2-NH2-based Poly-Pc composite using glutaraldehyde.
• Electrochemical measurements using differential pulse voltammetry (DPV) for PSA detection.

Main Results:

• The fabricated immunosensor demonstrated excellent analytical performance for PSA detection.
• Achieved a wide linear detection range from 0.01 to 1000 ng/mL with a low detection limit of 6.3 pg/mL.
• Exhibited high selectivity, reproducibility, and stability, even in complex synthetic serum samples.

Conclusions:

• The developed electrochemical immunosensor is a rapid, reliable, and sensitive tool for PSA detection.
• This technology shows significant potential for clinical diagnosis of prostate cancer.
• The immunosensor performance is comparable to established methods like ELISA.