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Updated: Sep 14, 2025

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Published on: April 28, 2021
Multiple functions of BRD4 in maintaining genomic stability in cancers
Zifeng Huang1, Tong Chu1, Wenzhe Ma1
1State Key Laboratory of Quality Research in Chinese Medicine & Faculty of Chinese Medicine, Macau University of Science and Technology, Macau SAR, 999078, China; Zhuhai MUST Science and Technology Research Institute, Macau University of Science and Technology, Hengqin Guangdong-Macao In-Depth Cooperation Zone, Guangdong, 519099, China.
None:
BRD4, renowned for its role in regulating oncogene expression through interactions with super-enhancers and promoters, plays a significant role in cancer progression. This critical function leads to considerable interest in the discovery, design, and synthesis of BRD4 inhibitors (BRD4i), a promising class of small molecule compounds that are currently advancing through various clinical trials. While the involvement of BRD4 in transcriptional regulation is well-established, recent studies illuminate its additional function in maintaining genomic stability through participation in an array of mechanisms underlying DNA damage response and repair, underscoring its multifaceted influence on cellular homeostasis. Capitalizing on this newfound knowledge, BRD4i are being explored in synergistic combinations with a range of DNA damage response targeted agents, thereby enhancing the efficacy of anti-cancer therapies. This study aims to provide a comprehensive overview of BRD4's contribution to various DNA damage-related mechanisms. By unraveling these complex interactions, this study aim to enhance our understanding of the multifaceted role of BRD4 in maintaining genomic stability but also to illuminate new avenues for therapeutic intervention.
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