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Transcriptomic profiling reveals sex-specific gene expression patterns in Eriocheir sinensis gonads.

Feng Zhao1, Haoyu Liu1, Yaxuan Shi1

  • 1College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Jiangsu Key Laboratory of Ocean-Land Environmental Change and Ecological Construction, Nanjing Normal University, Nanjing 210023, Jiangsu, China.

Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
|July 22, 2025
PubMed
Summary

This study identifies key genes and pathways involved in sex determination for the Chinese mitten crab (Eriocheir sinensis). Findings reveal molecular mechanisms regulating sexual development and provide targets for future research.

Keywords:
Eriocheir sinensisSex-related genesSexual regulationSteroid hormone

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Area of Science:

  • * Crustacean biology
  • * Molecular genetics
  • * Reproductive biology

Background:

  • * The Chinese mitten crab (Eriocheir sinensis) shows significant sexual dimorphism, crucial for its economic value.
  • * Molecular mechanisms of sex determination and regulation in this species are not well understood.

Purpose of the Study:

  • * To conduct the first comparative gonadal transcriptome analysis of Eriocheir sinensis.
  • * To identify genes and pathways involved in sex determination and regulation.

Main Methods:

  • * High-throughput sequencing of testicular and ovarian tissues.
  • * Differential gene expression analysis.
  • * Functional enrichment analysis and protein-protein interaction network construction.
  • * RT-qPCR for experimental validation.

Main Results:

  • * Identified 5894 differentially expressed genes (DEGs), with 2581 female-related and 3313 male-related.
  • * Revealed involvement of Steroid hormone biosynthesis and GnRH signaling pathways.
  • * Identified Estradiol 17-beta-dehydrogenase 8 (HSD17B8) and Heat shock protein 90 (HSP90) as hub genes.
  • * RT-qPCR confirmed transcriptomic data reliability.

Conclusions:

  • * This study provides a valuable gene resource for understanding sex determination in Eriocheir sinensis.
  • * Identified key molecular players and pathways regulating sexual development.