Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Catenins01:23

Catenins

2.4K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.4K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

2.9K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.9K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.4K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.4K
Structure of Cadherins01:25

Structure of Cadherins

3.6K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.6K
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

3.1K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
3.1K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.9K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oncogenic PIK3CA reprograms glutamine metabolism to drive bladder cancer progression.

Cell communication and signaling : CCS·2026
Same author

miR-486-5p and miR-144-3p as candidate regulators of cortisol biosynthesis: functional and transcriptomic evidence in adrenocortical cells.

European journal of endocrinology·2026
Same author

Distinct Genetic Alterations Drive Cushing Disease Versus Silent Corticotroph Adenomas.

The Journal of clinical endocrinology and metabolism·2026
Same author

Cigarette smoke induces FASN-dependent fatty acid metabolic rewiring to drive bladder cancer progression.

Cancer letters·2026
Same author

Hyaluronidase-1 mediates postprandial suppression of hepatic gluconeogenesis.

Life metabolism·2026
Same author

Efficient Method for High-Throughput Screening of Compound Libraries Targeting Human PD-L1.

ACS omega·2026

Related Experiment Video

Updated: Sep 14, 2025

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

12.2K

β-catenin functions as a molecular adapter for disordered cBAF interactions.

Yuen San Chan1, Qinyu Gao1, Sarah A Robinson1

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA; Center for Precision Environmental Health, Baylor College of Medicine, Houston, TX 77030, USA.

Molecular Cell
|July 22, 2025
PubMed
Summary

Scaffold proteins like beta-catenin act as molecular adapters, linking BAF (SWI/SNF) chromatin remodelers to transcription factors. This interaction is crucial for regulating gene accessibility and function in human cells.

Keywords:
IDRsadrenocortical carcinomachromatin remodelingco-activatorsscaffold proteinssteroid hormonestranscription factorstranscription regulatorsunstructured protein

More Related Videos

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

10.7K
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.6K

Related Experiment Videos

Last Updated: Sep 14, 2025

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
09:41

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Published on: June 17, 2014

12.2K
Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

10.7K
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

9.6K

Area of Science:

  • Molecular Biology
  • Chromatin Biology
  • Gene Regulation

Background:

  • BAF (SWI/SNF) complexes are critical chromatin remodelers that regulate DNA accessibility through interactions with various binding partners.
  • The precise mechanisms underlying BAF's interactions with diverse partners, particularly scaffold proteins, remain incompletely understood.

Purpose of the Study:

  • To investigate the role of scaffold proteins, specifically beta-catenin (CTNNB1), in mediating interactions between BAF complexes and transcription factors.
  • To elucidate the molecular basis for how beta-catenin bridges BAF and transcription factors like steroidogenic factor 1 (SF-1, NR5A1).

Main Methods:

  • Investigated the effects of BAF inhibition on SF-1/beta-catenin enhancer occupancy and target gene activation.
  • Utilized molecular visualization techniques to examine protein-protein interactions.
  • Assessed beta-catenin's role as an adapter linking BAF to multiple transcription factors including YAP1, SOX2, FOXO3, and CBP/p300.

Main Results:

  • BAF inhibition disrupted SF-1/beta-catenin enhancer occupancy and impaired SF-1 target gene activation.
  • Beta-catenin functions as a molecular adapter, connecting SF-1 to the intrinsically disordered region (IDR) of the BAF subunit ARID1A via its Armadillo repeats.
  • Beta-catenin was identified as a general adapter linking BAF to other factors, including YAP1, SOX2, FOXO3, and CBP/p300, through an IDR-mediated mechanism.

Conclusions:

  • Beta-catenin serves as a critical molecular adapter, facilitating the modular coordination of BAF chromatin remodelers with diverse transcription factors.
  • This adapter mechanism, involving beta-catenin and ARID1A's IDR, is essential for regulating gene accessibility and transcriptional outcomes.
  • The findings reveal a general principle of how intrinsically disordered regions and adapter proteins enable dynamic interactions within the BAF interactome.