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Updated: Sep 14, 2025

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Published on: May 11, 2020
The transcription factor StERF75 negatively regulates starch biosynthesis by targeting Isoamylase in potato
Ju Ai1, Meihong Yang1, Jianhua Zou1
1Key Laboratory for Potato Biology of Yunnan Province, The CAAS-YNNU-YINMORE Joint Academy of Potato Science, Yunnan Normal University, Kunming 650500, China.
Abstract:
Starch yield is closely related to the nutritional and economic value of potato. Transcriptional regulation of starch biosynthesis is an important process, however, it remains elusive in potato. An AP2/ERF transcription factor, StERF75, was responsive to sucrose and ABA stimuli, and its function in starch biosynthesis was characterized in this study. StERF75 contains two conserved domains, the AP2 domain and the EAR repression motif. It was highly expressed in tubers and localized in the cell nucleus. Among the examined eight promoters of starch synthesis-related genes, StERF75 specifically interacted with isoamylase1.1 (ISA1.1) promoter through cis-element ATCTA. StERF75 repressed ISA1.1 promoter and the EAR motif was required for the repression. Moreover, StNF-YC1 was found to interact with StERF75 and their co-expression enhanced the repressive effect on ISA1.1 activity. Overexpression of StERF75 resulted in reduced total starch content and altered physicochemical characteristics of tuber starch. This study reveals an important role of an EAR-motif repressor, StERF75, in starch biosynthesis, and provides a potential target gene to modulate starch in potato.
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