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Enhanced sensitivity in PCSK9 detection using binding-induced DNA walker-triggered Argonaute protein-based DNA
Xiaojuan Lin1, Shuhao Wang2, Xiaoling Liu1
1Department of Clinical Laboratory, The Central Hospital of Xingtai, Xingtai, 054000, Hebei, China.
Mikrochimica Acta
|July 22, 2025
Summary
A new DNA circuit method offers highly sensitive detection of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9). This biosensor approach provides a rapid, accurate, and stable tool for assessing cardiovascular disease risk in clinical settings.
Area of Science:
- Biomedical Engineering
- Molecular Diagnostics
- Cardiovascular Research
Background:
- Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) detection is crucial for lipid metabolism and cardiovascular disease risk assessment.
- Conventional methods like ELISA and Western blotting are sensitive but labor-intensive and time-consuming.
- Emerging biosensors offer faster, more sensitive, and cost-effective detection but face scalability challenges.
Purpose of the Study:
- To develop a novel, highly sensitive, and specific method for detecting PCSK9.
- To overcome the limitations of traditional PCSK9 detection techniques.
- To provide a promising tool for clinical PCSK9 monitoring.
Main Methods:
- Development of a novel sensing platform utilizing a binding-induced DNA walker.
- Integration of the DNA walker with a TtAgo-based DNA circuit for signal amplification.
- Validation of the method using serum samples for PCSK9 detection.
Main Results:
- Achieved a limit of detection as low as 2.5 fg/mL for PCSK9.
- Demonstrated excellent specificity and stability, retaining 93.1% signal after 15 days.
- Successfully detected PCSK9 in human serum samples with high accuracy.
Conclusions:
- The novel DNA walker-triggered TtAgo-based DNA circuit offers a sensitive and stable platform for PCSK9 detection.
- This method presents a significant advancement over traditional techniques for clinical applications.
- The developed biosensor shows strong potential for routine use in assessing cardiovascular risk.
