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CircRNA_26782 Regulates Axonal Growth via Inhibiting the Autophagy Pathway in a Rat Model of Spinal Cord Injury
Xiaowei Qian1,2, Wei Zhang1, Dexia Kong1
1Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, China.
Journal of Biochemical and Molecular Toxicology
|July 23, 2025
Summary
Circular RNAs (circRNAs) promote axonal growth in spinal neurons by inhibiting the miR-19b-2-5p/Rab1b-mediated autophagy pathway. This finding offers insights into spinal cord injury mechanisms and potential therapies.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Circular RNAs (circRNAs) are key regulators in biological processes.
- Spinal cord injury (SCI) involves complex molecular mechanisms affecting neuronal repair.
- Understanding circRNA roles in neuronal regeneration is crucial for therapeutic development.
Purpose of the Study:
- Investigate the function and mechanism of circRNA_26782 in spinal neurons.
- Determine the role of circRNA_26782 in a rat model of spinal cord injury.
- Elucidate the molecular pathway regulated by circRNA_26782 in axonal growth.
Main Methods:
- Differential circRNA expression profiling in a rat SCI model.
- Fluorescence in situ hybridization for subcellular localization.
- RNA sequencing to identify interacting microRNAs and target genes.
- In vitro assays for axonal length and autophagy pathway analysis.
Main Results:
- circRNA_26782 was downregulated in spinal cord injury.
- circRNA_26782 knockdown increased axonal length.
- circRNA_26782 regulates axonal growth via the miR-19b-2-5p/Rab1b axis.
- The miR-19b-2-5p/Rab1b axis modulates the autophagy pathway.
Conclusions:
- circRNA_26782 promotes axonal growth by suppressing the miR-19b-2-5p/Rab1b pathway, thereby inhibiting autophagy.
- This study reveals a novel circRNA-mediated mechanism for axonal regeneration.
- Findings provide potential therapeutic targets for spinal cord injury treatment.
