Circ_PSD3 Stimulates Cell Proliferation, Migration, Invasion and Epithelial to Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma via the Regulation of miR-145-5p/miR-338-3p/HMGB3 Axis
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Circular RNA circ_PSD3 promotes papillary thyroid carcinoma (PTC) progression by regulating the miR-145-5p/miR-338-3p/HMGB3 axis. This study identifies circ_PSD3 as a potential therapeutic target for PTC treatment.
Area Of Science
- Molecular Biology
- Oncology
- Biochemistry
Background
- Circular RNAs (circRNAs) are emerging as crucial regulators in various cancers, including papillary thyroid carcinoma (PTC).
- The specific role and mechanism of circ_PSD3 in PTC remain largely uncharacterized, necessitating further investigation.
- Understanding circRNA functions can lead to novel diagnostic biomarkers and therapeutic strategies for PTC.
Purpose Of The Study
- To elucidate the function and molecular mechanism of circ_PSD3 in papillary thyroid carcinoma (PTC).
- To investigate the regulatory axis involving circ_PSD3, microRNAs (miR-145-5p and miR-338-3p), and HMGB3 in PTC.
- To assess the potential of circ_PSD3 as a therapeutic target for PTC.
Main Methods
- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) to measure gene and miRNA expression levels.
- Cell counting kit-8 and Transwell assays to evaluate cell proliferation, migration, and invasion.
- Dual-luciferase reporter assays to confirm miRNA targeting, and Western blot to assess epithelial-mesenchymal transition (EMT) markers.
Main Results
- Circ_PSD3 and HMGB3 expression were significantly upregulated in PTC tissues and cells.
- Knockdown of circ_PSD3 or HMGB3 inhibited PTC cell proliferation, migration, and invasion.
- Circ_PSD3 directly targets miR-145-5p and miR-338-3p, which in turn target HMGB3; both miRNAs act as tumor suppressors in PTC.
Conclusions
- Circ_PSD3 promotes PTC cell proliferation, migration, invasion, and EMT by modulating the miR-145-5p/miR-338-3p/HMGB3 pathway.
- The circ_PSD3/miR-145-5p/miR-338-3p/HMGB3 axis represents a novel mechanism driving PTC progression.
- Circ_PSD3 holds promise as a potential diagnostic biomarker and therapeutic target for papillary thyroid carcinoma.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
Related Concept Videos
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...