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Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

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Medium-assisted tumbling controls bacteria exploration in a complex fluid.

Héctor Urra1, Thierry Darnige1, Xavier Benoit-Gonin1

  • 1Laboratoire PMMH-ESPCI Paris, PSL Research University, Sorbonne Université and Université Paris cité, 7, quai Saint-Bernard, Paris, France. hectorignaciou@gmail.com.

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Summary
This summary is machine-generated.

Bacterial exploration in non-Newtonian fluids is medium-assisted, not solely biologically driven. Local mechanical properties override E. coli

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Area of Science:

  • Microbiology
  • Rheology
  • Biophysics

Background:

  • Many natural fluids with bacteria exhibit non-Newtonian rheology.
  • Understanding bacterial spatial exploration in such complex media is crucial.

Purpose of the Study:

  • To investigate how E. coli's motility is affected by tunable macroscopic rheology.
  • To characterize bacterial navigation in Newtonian versus yield-stress fluids.

Main Methods:

  • Designed a motility medium with tunable rheology using carbomer grains.
  • Employed 3D Lagrangian tracking to analyze individual bacterial trajectories.
  • Compared wild-type E. coli with a smooth runner mutant.

Main Results:

  • Observed changes in swimming speed, persistence, and diffusivity with increasing carbomer concentration.
  • Identified a motility barrier at higher concentrations.
  • Demonstrated that local mechanical disorder and resistance override run-and-tumble navigation.

Conclusions:

  • Bacterial exploration in non-Newtonian fluids is significantly influenced by medium properties.
  • A "medium-assisted" exploration scenario with stop-and-go kinematics was characterized.
  • Flagellar bundle flexibility is linked to this medium-assisted movement.