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Rat Model of Granulomatous Mastitis
Jie Gong1,2, Juan Wang1,2, Xin Zhong1,2
1Department of Galactophore, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410007, People's Republic of China.
Journal of Inflammation Research
|July 23, 2025
Summary
Researchers developed a new rat model for granulomatous mastitis (GM). This model accurately mimics human GM, aiding future research into this immune-related disease.
Area of Science:
- Veterinary Medicine
- Immunology
- Pathology
Background:
- Granulomatous mastitis (GM) is an immune-related condition that is difficult to treat.
- Lack of suitable animal models has hindered research into GM's mechanisms and therapies.
Purpose of the Study:
- To establish a stable and reproducible rat model for granulomatous mastitis (GM).
- To facilitate future translational research and therapeutic development for GM.
Main Methods:
- Rats were injected with varying doses of tissue homogenate-complete Freund's adjuvant (CFA) suspension.
- The optimal dose (0.4 mL) was determined by evaluating gross morphology, inflammation index, and histopathology.
- Inflammatory marker expression (TNF-α, IL-1β, IFN-γ, IL-2) was assessed over 28 days.
Main Results:
- The 0.4 mL dose successfully induced GM-like symptoms, including swelling, ulceration, and fistula formation.
- Histopathology revealed granulomas and persistent inflammatory cell infiltration.
- Key inflammatory factors (IL-2, IL-1β, TNF-α, IFN-γ) were significantly elevated in the model group.
Conclusions:
- A 0.4 mL tissue homogenate-CFA injection reliably creates a stable rat model of GM.
- This model closely replicates human GM, offering a valuable platform for further investigation.
