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Updated: Jul 17, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
Enhanced glass ionomer cement with bioactive additives for collagen synthesis and inflammation in pulp-rabbit teeth
Nattapon Rotpenpian1, Peerapon Sornying2, Sareepah Manmoo2
1Department of Oral Biology and Occlusion, Faculty of Dentistry, Prince of Songkla University, HatYai, 90110, Thailand.
Objective:
This study aimed to enhance conventional glass ionomer cement (GIC) by incorporating 15 % chitosan, 5 % bovine serum albumin (BSA), 0.05 % tricalcium phosphate (TCP), and 1 μg translationally controlled tumor protein (TCTP), resulting in an enhanced-GIC formulation. The study evaluated its adhesion properties, biocompatibility, and ability to promote pulp tissue healing in rabbit anterior teeth.
Materials And Methods:
The enhanced-GIC was tested in a rabbit model to assess its physical adhesion and biological effects on pulp tissue. Following cavity preparation and material placement, the teeth were observed for 21 days. Histological evaluations focused on inflammation, toxicity, and collagen synthesis in pulp tissue.
Result:
The enhanced-GIC showed comparable adhesion properties to conventional GIC. Histological analysis revealed no significant inflammation or toxicity in the pulp tissue of either group. The enhanced-GIC group exhibited superior biocompatibility, demonstrated by increased lymphocyte infiltration and enhanced collagen synthesis within the pulp tissue, suggesting its potential for promoting tissue regeneration.
Conclusion:
The enhanced-GIC formulation shows promise as a sublining material in restorative dentistry, offering benefits in pulp tissue healing and collagen formation, while maintaining adhesion comparable to conventional GIC. This study highlights enhanced-GIC's potential for use in dental restorative procedures, particularly for cases requiring pulp regeneration.
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