Related Experiment Video For Cancer
Updated: Sep 14, 2025

09:49
Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Published on: July 5, 2019
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Detection of Cancer-Associated Gene Fusion by Ligation-Dependent RT-PCR
Vinciane Rainville1, Marie-Delphine Lanic1, Fanny Drieux1,2
1INSERM U1245, Centre Henri Becquerel, University of Rouen, Rouen, France.
Methods in Molecular Biology (Clifton, N.J.)
|July 23, 2025
Abstract:
Ligation-mediated RT-PCR is a rapid and highly sensitive solution to detect the presence of gene fusions in cancers. Here, we provide a detailed protocol which allows the detection of hundreds of these tumor markers in a single assay, in a rapid turnaround time.

