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Published on: July 5, 2019
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Detection of Cancer-Associated Gene Fusion by Ligation-Dependent RT-PCR
Vinciane Rainville1, Marie-Delphine Lanic1, Fanny Drieux1,2
1INSERM U1245, Centre Henri Becquerel, University of Rouen, Rouen, France.
Methods in Molecular Biology (Clifton, N.J.)
|July 23, 2025
Summary
Ligation-mediated RT-PCR offers a fast and sensitive method for detecting numerous cancer gene fusions. This protocol enables the identification of hundreds of tumor markers in a single, rapid assay.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Gene fusions are critical drivers in cancer development.
- Accurate detection of gene fusions is essential for diagnosis and targeted therapy.
- Current methods for detecting gene fusions can be time-consuming or lack sensitivity.
Purpose of the Study:
- To present a detailed protocol for ligation-mediated RT-PCR.
- To enable the detection of hundreds of cancer-specific gene fusions.
- To achieve rapid turnaround time for tumor marker identification.
Main Methods:
- Utilizing ligation-mediated reverse transcription polymerase chain reaction (RT-PCR).
- Designing a single assay capable of detecting multiple gene fusions.
- Implementing a protocol for high-throughput analysis of tumor markers.
Main Results:
- The protocol allows for the detection of hundreds of gene fusions.
- The assay demonstrates high sensitivity in identifying tumor markers.
- The method provides a rapid turnaround time for results.
Conclusions:
- Ligation-mediated RT-PCR is a powerful tool for comprehensive cancer gene fusion detection.
- This protocol facilitates efficient and sensitive identification of numerous tumor markers.
- The rapid nature of the assay supports timely clinical decision-making in oncology.
