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Updated: Sep 14, 2025

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
Targeted 16S rRNA Gene Sequencing for Water Samples
Mélanie Pimenta1, Margaux Gaschet1, Sylvain Meyer2
1Univ. Limoges, RESINFIT, INSERM U1092, Limoges, France.
Abstract:
The choice of variable region to amplify in 16S rRNA-targeted amplicon sequencing has long been a matter of debate. Here, we describe a method for sequencing multiple variable regions with the Ion 16S Metagenomics kit, which amplifies six different amplicons covering seven 16S variable regions. We present the full protocol of water sequencing from sample collection to library preparation.
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