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Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of

Tim Hohmann1, Urszula Hohmann1, Faramarz Dehghani1

  • 1Department of Anatomy and Cell Biology, Medical Faculty, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle (Saale), Germany.

Current Issues in Molecular Biology
|July 23, 2025
PubMed

Insights

RNA modifications like pseudouridinylation and m6A methylation are crucial in cancer. This study links pseudouridine synthase 7 (PUS7) and WT1-associated protein (WTAP) to renal cell carcinoma (RCC) progression and survival.

Area of Science:

  • Molecular Biology
  • Oncology
  • Epigenetics

Background:

  • RNA modifications are increasingly recognized for their role in cancer development.
  • Key modifications include pseudouridinylation and N6-methyladenosine (m6A) methylation.
  • These modifications impact essential cellular processes like proliferation and survival.

Purpose of the Study:

  • To investigate the role of RNA-modifying factors pseudouridine synthase 7 (PUS7) and WT1-associated protein (WTAP) in renal cell carcinoma (RCC).
  • To correlate PUS7 and WTAP expression with tumor markers, immune checkpoints, and patient survival.
  • To identify novel mRNA targets regulated by PUS7 and WTAP in RCC.

Main Methods:

  • Comparative analysis of RNA-modifying factors PUS7 and WTAP in human RCC tumor data.
  • Statistical correlation analysis with proliferation markers (CXCR4, TP53, PTEN, NRAS) and immune checkpoints (HLA-G, LGALS9).
  • Identification of PUS7 and WTAP target mRNAs using bioinformatics approaches.

Main Results:

  • PUS7 and WTAP expression significantly correlated with proliferation and prognosis markers in RCC.
  • Both factors showed a significant association with overall survival in RCC patients.
  • Identified target mRNAs involved in mitosis, cell cycle, cell division, and the WNT pathway.

Conclusions:

  • PUS7 and WTAP are important RNA-modifying factors implicated in RCC pathogenesis.
  • These factors influence key cellular processes and patient outcomes in renal cell carcinoma.
  • Targeting PUS7 and WTAP may offer novel therapeutic strategies for RCC.
Keywords:
PUS7RCCRNA modificationsWTAPalternative splicingtranscriptome

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