Related Experiment Video
Updated: Sep 14, 2025
06:01
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
Published on: June 7, 2016
6.9K
Role of Micro RNA Expression as Biomarker in Breast Cancer
Gitika Nanda Singh1, Nijarth Shahi1, Parijat Suryavanshi1
1Department of General Surgery, King George's Medical University, Lucknow, Uttar Pradesh, India.
Annals of African Medicine
|July 23, 2025
Summary
MicroRNA-21 (miR-21) is elevated in breast cancer (BC) patients, correlating with advanced stages and lymph node involvement. This finding highlights miR-21 as a potential biomarker for diagnosing and predicting BC outcomes.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Breast cancer (BC) is the most common malignancy in women.
- MicroRNA-21 (miR-21) is an oncomiR found at elevated levels in various cancers, including BC.
- MicroRNAs regulate critical cancer processes like apoptosis, invasion, metastasis, and proliferation.
Purpose of the Study:
- To investigate the molecular basis of miR-21 in breast cancer.
- To determine the clinical significance of miR-21 as a biomarker in BC.
Main Methods:
- Quantitative polymerase chain reaction (qPCR) with Taq-Man probe was used to analyze miR-21 levels.
- Real-time polymerase chain reaction and reverse transcription (RT) were performed.
- Relative quantification of gene expression (fold change) was calculated using RT-qPCR data.
Main Results:
- Serum miR-21 levels were significantly higher in BC cases (8.17 ± 2.49) compared to controls (1.02 ± 0.23).
- Elevated miR-21 levels correlated with advanced BC stages (III, IV) and lymph node metastasis.
- miR-21 expression showed a significant association with Ki67 protein levels.
Conclusions:
- Plasma miR-21 levels serve as a valuable diagnostic biomarker for breast cancer.
- miR-21 is a significant prognostic biomarker for predicting breast cancer outcomes.
