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Updated: May 8, 2026
A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain
Published on: March 7, 2012
Protocol for synthesizing, implanting, and using polydimethylsiloxane as skull replacement in mice for imaging,
Kengo Takahashi1, Gerjan Huis In 't Veld2, Davide Benedetti3
1Swammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Amsterdam Brain and Cognition, University of Amsterdam, 1098XH Amsterdam, the Netherlands.
Abstract:
Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain are now available. However, few effective approaches enable both optical and mechanical access to the brain. Here, we present a protocol for synthesizing, implanting, and using polydimethylsiloxane (PDMS) windows as skull replacements for chronic wide-field and two-photon calcium imaging in mice. We also describe steps for performing viral injections and multi-site silicon probe implantation.
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