Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancer Therapies02:49

Cancer Therapies

8.0K
Cancer therapies are various modes of treatment, such as surgery, radiation therapy, and chemotherapy that are administered to cancer patients.
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...
8.0K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

5.9K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.9K
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

1.4K
Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
1.4K
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

15.0K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
15.0K
Cancer Prevention02:59

Cancer Prevention

6.3K
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
6.3K
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

3.1K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural Analysis and Substrate Specificity of D-Carbamoylase from <i>Pseudomonas</i>.

Biotech (Basel (Switzerland))·2024
Same author

Targeted Strategies for Degradation of Key Transmembrane Proteins in Cancer.

Biotech (Basel (Switzerland))·2023
Same author

Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery.

Biotech (Basel (Switzerland))·2022
Same author

Reactive Chemicals and Electrophilic Stress in Cancer: A Minireview.

High-throughput·2018

Related Experiment Video

Updated: Sep 14, 2025

A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke
09:23

A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke

Published on: January 2, 2012

19.5K

Reactive Molecules in Cigarette Smoke: Rethinking Cancer Therapy.

Vehary Sakanyan1

  • 1Faculty of Biology, Faculty of Pharmacy, Nantes University, 44035 Nantes, France.

Biotech (Basel (Switzerland))
|July 23, 2025
PubMed
Summary

Detecting reactive oxygen species (ROS) in tobacco smoke aids precision cancer therapy. Smoking cessation remains the most effective cure for tobacco-related cancers, aligning with a "one-cure" approach.

Keywords:
cancer progressionelectrophilic moleculesin-gel detectionreactive oxygen species (ROS)regular cigarettesmoking cessation

More Related Videos

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
09:25

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Published on: January 10, 2015

11.5K
Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
10:47

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

Published on: February 21, 2011

34.3K

Related Experiment Videos

Last Updated: Sep 14, 2025

A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke
09:23

A Protocol for Detecting and Scavenging Gas-phase Free Radicals in Mainstream Cigarette Smoke

Published on: January 2, 2012

19.5K
Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
09:25

Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations

Published on: January 10, 2015

11.5K
Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
10:47

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

Published on: February 21, 2011

34.3K

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Reactive oxygen species (ROS) in tobacco smoke are implicated in cancer development.
  • Understanding superoxide production by electrophilic molecules is crucial.
  • Hydrogen peroxide's dual role in oxidative stress presents therapeutic targets.

Purpose of the Study:

  • To explore mechanisms of anti-ROS action for cancer therapy in smokers.
  • To investigate the interaction of ROS and electrophilic compounds with proteins.
  • To identify potential protein targets for inhibiting ROS-associated cancer progression.

Main Methods:

  • Utilizing fluorescent protein detection in living cells (in-gel).
  • Analyzing rapid interactions between ROS, electrophilic compounds, and targeted proteins.

Main Results:

  • Advances in ROS detection and understanding superoxide production.
  • Identification of potential protein targets for cancer therapy.
  • Limitations in developing specific ROS inhibitors for smokers.

Conclusions:

  • Current pharmaceutical approaches may not fully address tobacco-induced cancers.
  • Smoking cessation is the most effective strategy for combating tobacco-related cancer.
  • A "one-cure" paradigm focusing on cessation is highlighted.