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Proteoform Patterns in Hepatocellular Carcinoma Tissues: Aspects of Oncomarkers

  • 0Institute of Biomedical Chemistry, Pogodinskaya, 10, Moscow 119121, Russia.
Proteomes +

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July 23, 2025

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July 23, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Proteomics reveals distinct protein modifications (proteoforms) in hepatocellular carcinoma (HCC) compared to normal liver tissue. These proteoform patterns offer potential new biomarkers for early cancer detection and diagnosis.

Area Of Science

  • Proteomics and Cancer Biology
  • Biomarker Discovery

Background

  • Human proteins exhibit diverse modifications, known as proteoforms, which are crucial for biomarker development.
  • Hepatocellular carcinoma (HCC) presents a significant global health challenge, necessitating novel diagnostic approaches.

Purpose Of The Study

  • To compare proteoform patterns between HCC and nonmalignant liver tissues.
  • To identify potential proteomic biomarkers for HCC detection.

Main Methods

  • Utilized a top-down proteomics strategy involving two-dimensional gel electrophoresis (2DE).
  • Integrated LC-ESI-MS/MS for comprehensive protein profile and proteoform analysis.
  • Analyzed proteoform patterns in both cancerous and non-cancerous liver samples.

Main Results

  • Visualized over 2500 proteoform patterns per sample, distinguishing malignant from nonmalignant liver cells.
  • Identified 1270 protein patterns consistently present across all samples.
  • Found 38 proteins, including PKM and ANXA2, with significantly different proteoform patterns in HCC.

Conclusions

  • While most proteoforms are conserved, altered patterns and novel proteoforms emerge in HCC.
  • These distinct proteoforms represent promising candidates for further investigation as HCC biomarkers.
  • Prioritized specific proteins with altered proteoform profiles for in-depth characterization.

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