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Updated: Sep 14, 2025

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Published on: June 2, 2021
Golgin Protein MoCoy1 Mediates Retrograde Trafficking From the Golgi to the ER, Regulating Fungal Development and
Xiaoru Kang1,2, Jianyu Si1,2, Na Li1,2
1Department of Plant Pathology, College of Plant Protection, Anhui Agricultural University, Hefei, China.
Abstract:
The Golgi apparatus is a vital organelle involved in protein sorting and trafficking. Golgins, a family of coiled-coil proteins, play an important role in maintaining the structure and function of the Golgi apparatus in eukaryotes. However, the function of golgins in the plant-pathogenic fungus Magnaporthe oryzae remains uncharacterised. Here, we systematically investigated the biological role of the golgin protein MoCoy1 in M. oryzae. Our results show that MoCoy1 is primarily localised to the Golgi apparatus. MoCOY1 deletion led to defects in vegetative growth, conidiation and pathogenicity of M. oryzae. In addition, MoCoy1 affected the secretion of the cytoplasmic effector proteins. Furthermore, MoCoy1 interacted with retrograde Golgi-related components and affected the retrograde transport from the Golgi to the endoplasmic reticulum (ER). Overall, our findings suggest that the golgin protein MoCoy1 mediates ER-Golgi retrograde trafficking, thereby regulating the development and pathogenicity of M. oryzae.
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