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Updated: Sep 14, 2025

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds
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Tenascin-c functionalised self-assembling peptide hydrogels for critical-sized bone defect reconstruction.

Alexandre Trubert-Paneli1, Jonathan A Williams2, James F C Windmill3

  • 1Centre for the Cellular Microenvironment, Mazumdar-Shaw Advanced Research Centre, University of Glasgow, Glasgow, United Kingdom.

Biomaterials
|July 23, 2025
PubMed
Summary
This summary is machine-generated.

This study developed a tenascin-c functionalized peptide hydrogel to treat critical-sized bone defects. The biomaterial effectively retained BMP-2, promoting bone regeneration and complete healing in mice.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Engineering

Background:

  • Critical-sized bone defects pose significant clinical challenges due to limited healing capacity.
  • Current bone regeneration strategies using bone morphogenetic protein-2 (BMP-2) face limitations, including uncontrolled protein release and adverse effects.
  • Extracellular matrix proteins offer a promising approach to enhance biomaterial performance for bone regeneration.

Purpose of the Study:

  • To create and evaluate a novel tenascin-c functionalized self-assembling peptide hydrogel for regenerating critical-sized bone defects.
  • To assess the hydrogel's ability to control BMP-2 release and promote osteogenic differentiation.
  • To investigate the in vivo efficacy of the functionalized hydrogel in promoting bone defect healing.

Main Methods:

  • Fabrication of a peptide hydrogel functionalized with a recombinant tenascin-c fragment (domains 3-5).
  • Incorporation of BMP-2 into the tenascin-c functionalized hydrogel.
  • In vitro assessment of BMP-2 retention and mesenchymal stem cell (MSC) osteogenic differentiation.
  • In vivo evaluation of the hydrogel's efficacy in healing critical-sized murine bone defects.

Main Results:

  • The tenascin-c functionalized hydrogel effectively retained BMP-2.
  • The construct successfully induced osteogenic differentiation of MSCs.
  • Complete unionization of critical-sized bone defects was achieved in vivo using a low dose of BMP-2.
  • Tenascin-c functionalization mitigated adverse effects associated with uncontrolled BMP-2 release.

Conclusions:

  • Tenascin-c is a suitable candidate for functionalizing biomaterials in bone engineering applications.
  • Self-assembling peptide hydrogels functionalized with tenascin-c show significant potential for treating critical-sized bone defects.
  • This approach offers a promising strategy for controlled delivery of growth factors to enhance bone regeneration.