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Related Experiment Video

Updated: Jul 25, 2026

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Published on: December 10, 2010

Tenascin-c functionalised self-assembling peptide hydrogels for critical-sized bone defect reconstruction.

Alexandre Trubert-Paneli¹, Jonathan A Williams², James F C Windmill³

¹Centre for the Cellular Microenvironment, Mazumdar-Shaw Advanced Research Centre, University of Glasgow, Glasgow, United Kingdom.

|July 23, 2025

Summary

This study developed a tenascin-c functionalized peptide hydrogel to treat critical-sized bone defects. The biomaterial effectively retained BMP-2, promoting bone regeneration and complete healing in mice.

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Published on: September 11, 2015

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

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Last Updated: Jul 25, 2026

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects

Published on: December 10, 2010

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

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Published on: September 11, 2015

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

Published on: June 13, 2018

Area of Science:

Biomaterials Science
Regenerative Medicine
Orthopedic Engineering

Background:

Critical-sized bone defects pose significant clinical challenges due to limited healing capacity.
Current bone regeneration strategies using bone morphogenetic protein-2 (BMP-2) face limitations, including uncontrolled protein release and adverse effects.
Extracellular matrix proteins offer a promising approach to enhance biomaterial performance for bone regeneration.

Purpose of the Study:

To create and evaluate a novel tenascin-c functionalized self-assembling peptide hydrogel for regenerating critical-sized bone defects.
To assess the hydrogel's ability to control BMP-2 release and promote osteogenic differentiation.
To investigate the in vivo efficacy of the functionalized hydrogel in promoting bone defect healing.

Main Methods:

Fabrication of a peptide hydrogel functionalized with a recombinant tenascin-c fragment (domains 3-5).
Incorporation of BMP-2 into the tenascin-c functionalized hydrogel.
In vitro assessment of BMP-2 retention and mesenchymal stem cell (MSC) osteogenic differentiation.
In vivo evaluation of the hydrogel's efficacy in healing critical-sized murine bone defects.

Main Results:

The tenascin-c functionalized hydrogel effectively retained BMP-2.
The construct successfully induced osteogenic differentiation of MSCs.
Complete unionization of critical-sized bone defects was achieved in vivo using a low dose of BMP-2.
Tenascin-c functionalization mitigated adverse effects associated with uncontrolled BMP-2 release.

Conclusions:

Tenascin-c is a suitable candidate for functionalizing biomaterials in bone engineering applications.
Self-assembling peptide hydrogels functionalized with tenascin-c show significant potential for treating critical-sized bone defects.
This approach offers a promising strategy for controlled delivery of growth factors to enhance bone regeneration.