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Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

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Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
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Related Experiment Video

Updated: Sep 14, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
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Polypeptide Single-Chain Nanoparticle Derived Artificial Enzyme.

Yongjia Yang1, Hesong Wang2, Song Hong3

  • 1Beijing Area Major Laboratory of Peptide and Small Molecular Drugs, School of Pharmaceutical Sciences, Capital Medical University, Beijing, 100069, China.

Advanced Healthcare Materials
|July 24, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed artificial enzymes using self-folding polypeptides and metal oxide nanoclusters. These novel biomimetic catalysts exhibit enzyme-like activity, offering potential therapeutic applications for oxidative stress and inflammation.

Keywords:
artificial proteinenzymenanozymepolypeptidesuperoxide dismutase

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Area of Science:

  • Biomaterials Science
  • Catalysis
  • Nanotechnology

Background:

  • Polyamino acids from ring-opening polymerization (ROP) of N-carboxyanhydride (NCA) monomers are scalable but lack sequence specificity for enzyme-like function.
  • Natural enzymes achieve complex functions through precise folding, a capability limited in synthetic polypeptides.

Purpose of the Study:

  • To engineer artificial enzymes with enzyme-like activity and controlled folding.
  • To utilize metal oxide nanoclusters as coenzymes within a polypeptide framework.
  • To investigate the therapeutic potential of these artificial enzymes in an inflammatory disease model.

Main Methods:

  • Synthesis of a triblock copolypeptide (PEG-b-PGlu-b-P(Glu-EG2)) via ROP of NCAs.
  • Intramolecular cross-linking of the central poly-Glu block using transition metal ions (Fe, Mn, Co, Cu, Zn).
  • Formation of metal/alloy oxide nanoclusters within the polypeptide via co-precipitation.
  • Characterization of structural transitions (α-helix to β-strand) and catalytic activities (POD, SOD).

Main Results:

  • Successfully created single-chain nanoparticles (SCNPs) with cross-linked poly-Glu domains containing metal oxide nanoclusters.
  • Demonstrated metal-specific peroxidase (POD) and superoxide dismutase (SOD) activities.
  • FeOx-complexed artificial enzymes (Fe-enzymes) showed therapeutic effects in a rheumatoid arthritis mouse model by mitigating oxidative stress and inflammation.
  • Artificial enzymes exhibited high activity with low metal content (< 5 wt.%) and protein-like secondary structures.

Conclusions:

  • Developed a novel strategy for creating artificial enzymes with tunable catalytic activities and biomimetic folding.
  • Metal oxide nanoclusters within polypeptide SCNPs effectively act as coenzymes.
  • These artificial enzymes hold promise for therapeutic applications targeting oxidative stress-related diseases.