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Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions

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Published on: July 22, 2013

Microtomy-fabricated two-dimensional nano-slits enable single molecule biosensing.

Muhammad Sajeer P^1,2,3,4, Ankit Bhardwaj^2,4, Boya Radha^2,4,5

¹Department of Chemistry, School of Natural Sciences, The University of Manchester, Manchester M13 9PL, UK. ashok.keerthi@manchester.ac.uk.

|July 24, 2025

Summary

We developed a scalable fabrication method for atomically smooth 2D nano-slits using ultramicrotomy. This technique enables mass production of identical biosensors for sensitive DNA detection and analysis of DNA conformations.

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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Published on: September 19, 2017

Area of Science:

Materials Science
Nanotechnology
Biosensing

Background:

Biological nanopores excel in single-molecule sensing, but solid-state counterparts lag due to fabrication challenges.
Lack of scalable, precise manufacturing limits solid-state nanopore adoption in molecular sensing.
Producing identical solid-state devices with atomic precision is a significant barrier.

Purpose of the Study:

To demonstrate a novel, scalable fabrication method for solid-state nano-slit biosensors.
To enable precise, high-throughput production of identical nano-slit devices.
To apply these nano-slits for sensitive DNA detection and conformational analysis.

Main Methods:

Ultramicrotomy-based fabrication of atomically smooth 2D nanocapillaries.
Utilizing molybdenum disulfide (MoS2) layered crystals for nano-slit creation.
Resistive ionic current blockade technique for DNA sensing.

Main Results:

Sustainable production of hundreds of identical MoS2 2D nano-slits with tunable dimensions.
Demonstrated sensitive DNA detection using the fabricated nano-slits.
Analyzed DNA translocation dynamics and topological conformations via 1/f noise analysis.

Conclusions:

Ultramicrotomy offers a robust and scalable solution for fabricating identical solid-state nano-slit biosensors.
This method significantly accelerates device production compared to previous techniques.
The MoS2 nano-slits show great promise for advanced molecular sensing applications.