Extending the Range of Sizes of Monodisperse Core-Shell Hydrogel Capsules from Composite Jet Breakup by Combined...

The production of monodisperse particles or droplets is a longstanding issue across various fields, from aerosol science to inkjet printing. In bioengineering, sub-millimeter cell-laden hydrogel capsules have proven valuable for developing in vitro tissue models. A common practical approach for producing such droplets relies on the Plateau-Rayleigh instability to break up a liquid compound jet in air. However, while the droplet size is closely linked to nozzle dimensions, achieving high monodispersity suitable for quantitative biological assays remains challenging due to coalescence events associated with the beads-on-a-string morphology of viscoelastic jets. Here, a microfluidic strategy is introduced, combining electrical and mechanical actuation to enhance control and versatility over jet breakup. By fine-tuning the excitation frequency to select specific modes and applying an electric potential to regulate coalescence, a phase diagram is established, enabling the generation of monodisperse droplets over a broad size range. Notably, a previously hidden effect of the electric field on jet behavior is uncovered and quantitatively characterized. Finally, after crosslinking the compound droplets, capsules with a hydrogel envelope and a core composed of a cell suspension are formed in conditions compatible with cell proliferation, which lay the groundwork for quantitative high precision biological assays.