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Updated: Sep 14, 2025

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

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Genomic and Phenotypic Characterization of Pseudomonas juntendi Strain A3e, a Drug-Resistant Isolate from Irrigation

Ángel Ibarra-Rodríguez1, Jean Pierre González-Gómez1, Manuel Ángel Castillo-Olea1

  • 1Laboratorio Nacional para la Investigación en Inocuidad Alimentaria (LANIIA), Centro de Investigación en Alimentación y Desarrollo, A.C. (CIAD), Carretera a Eldorado km 5.5, Campo El Diez, 80110, Culiacan, Sinaloa, Mexico.

Current Microbiology
|July 24, 2025
PubMed

Insights

This study characterizes Pseudomonas juntendi strain A3e from irrigation water, revealing multi-drug resistance and significant virulence factors. The findings highlight potential clinical risks from environmental bacteria.

Area of Science:

  • Microbiology
  • Genomics
  • Environmental Science

Background:

  • Pseudomonas juntendi is an emerging opportunistic pathogen known for multi-drug resistance.
  • This bacterium is frequently identified in clinical environments.

Purpose of the Study:

  • To characterize the P. juntendi strain A3e isolated from irrigation water.
  • To analyze its antimicrobial resistance and virulence potential through genomic and phenotypic methods.

Main Methods:

  • Antimicrobial susceptibility testing and whole-genome sequencing were employed.
  • Multi-locus sequence typing and average nucleotide identity analysis confirmed taxonomic classification.
  • Phenotypic assays assessed biofilm formation.

Main Results:

  • Strain A3e was confirmed as P. juntendi with >99% similarity.
  • The strain exhibited strong biofilm formation and resistance to multiple antibiotics.
  • Genomic analysis revealed 24 antibiotic-resistance genes and 50 virulence factors.

Conclusions:

  • This is the first genome of P. juntendi reported in Mexico.
  • Environmental P. juntendi strains possess significant antimicrobial resistance and virulence traits.
  • The potential clinical implications of environmental isolates warrant further attention.

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