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Fecal Microbiota Transplantation via Colonoscopy for Recurrent C. difficile Infection
Published on: December 8, 2014
Microbiota Transplantation Among Patients Receiving Long-Term Care: The Sentinel REACT Nonrandomized Clinical Trial
Michael H Woodworth1, Ahmed Babiker1, Radhika Prakash-Asrani1
1Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia.
Importance:
Intestinal multidrug-resistant organism (MDRO) colonization is highly prevalent in long-term acute care hospital (LTACH) patients and is associated with MDRO infection and transmission. However, there are no therapies approved by the US Food and Drug Administration to reduce intestinal MDRO colonization.
Objective:
To determine the safety and acceptability of fecal microbiota transplantation (FMT) in LTACH patients.
Design, Setting, And Participants:
This single-center, open-label nonrandomized clinical trial was conducted from April to December 2023 at an LTACH in the Southeastern US with median 50-patient census and 28-day length of stay. Patients with MDRO colonization were identified by perirectal prevalence sampling. Patients colonized with at least 1 target MDRO were approached for informed consent for FMT. FMT recipients were compared with untreated controls with MDRO colonization. Data were analyzed from August 2024 to May 2025.
Intervention:
Healthy donor fecal microbiota (50-100 g stool and 250 mL normal saline with 9% glycerol) instilled via gastrostomy tube or enema without antibiotic or bowel preparation conditioning.
Main Outcomes And Measures:
The primary outcome was frequency and severity of adverse events. Solicited adverse events were recorded for 7 days. Unsolicited adverse events were recorded for 6 months. Four weekly perirectal MDRO cultures were performed after FMT.
Results:
A total of 42 patients, including 10 (mean [SD] age, 63.8 (14.5) years; 7 [70%] female) who received FMT and 32 contemporaneous controls (mean [SD] age, 64.0 [13.7] years; 13 [41%] female) were assessed. In 2 prevalence surveys, 23 of 32 (72%) and 26 of 34 (77%) perirectal cultures grew at least 1 MDRO. Among the FMT group, 5 patients received FMT via gastrostomy alone, 4 via enema alone, and 1 with both routes more than 30 days apart. No serious adverse events were attributed to FMT, and post-FMT solicited adverse events were mild. At final visit, all perirectal cultures from FMT recipients grew at least 1 MDRO. Post hoc analyses found numerically fewer FMT recipients had positive blood culture results (0 individuals vs 6 individuals [19%]; P = .31), pathogen intestinal dominance (2 of 8 individuals [25%] vs 4 of 8 individuals [50%]; P = .61), and 7 fewer days of antibiotic therapy per 1000 patient days (median [IQR], 12.6 [0-25.2] days vs 19.7 [6.5-36.1] days; P = .38) compared with controls in the 6 months after prevalence survey, although these differences were not statistically significant. Accounting for higher baseline FMT recipient antibiotic use, difference-in-differences analysis estimated 26 (95% CI, -64 to 12) fewer days of antibiotic therapy per 1000 patient-days after FMT, although this difference was also not statistically significant.
Conclusions And Relevance:
In this nonrandomized pilot clinical trial, FMT was acceptable for LTACH patients without related serious adverse events. Although not powered to test these outcomes, this study found potential reductions in bacteremia, intestinal pathogen domination, and antibiotic use associated with FMT, suggesting FMT should be evaluated in larger, randomized trials.
Trial Registration:
ClinicalTrials.gov Identifier: NCT05780801.
Insights
Fecal microbiota transplantation (FMT) appears safe and acceptable for long-term acute care hospital patients with multidrug-resistant organisms (MDROs). Further research is needed to confirm potential benefits in reducing MDRO colonization and antibiotic use.
Area of Science:
- Infectious Diseases
- Gastroenterology
- Microbiology
Background:
- Intestinal multidrug-resistant organism (MDRO) colonization is common in long-term acute care hospital (LTACH) patients, increasing infection and transmission risks.
- Current FDA-approved therapies to reduce intestinal MDRO colonization are lacking.
Purpose of the Study:
- To assess the safety and acceptability of fecal microbiota transplantation (FMT) in LTACH patients colonized with MDROs.
Main Methods:
- A single-center, open-label, nonrandomized clinical trial involving 42 LTACH patients (10 FMT recipients, 32 controls).
- FMT was administered via gastrostomy tube or enema without antibiotic or bowel preparation.
- Safety was assessed via adverse events for up to 6 months; MDRO colonization was monitored via perirectal cultures.
Main Results:
- FMT was safe and well-tolerated, with no serious adverse events attributed to the procedure; mild solicited adverse events were reported.
- While all FMT recipients still had MDROs at the final visit, post hoc analyses suggested potential numerical reductions in bacteremia, intestinal pathogen dominance, and antibiotic use compared to controls.
- These observed differences did not reach statistical significance, likely due to the study's limited sample size.
Conclusions:
- Fecal microbiota transplantation (FMT) is a safe and acceptable intervention for LTACH patients with MDRO colonization.
- Preliminary findings suggest potential benefits of FMT in reducing bacteremia, pathogen dominance, and antibiotic usage.
- Larger, randomized controlled trials are warranted to definitively evaluate the efficacy of FMT in this patient population.
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