Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterization of conserved residues in the mammarenavirus matrix protein Z using novel Lassa virus life cycle modelling assays.

Scientific reports·2026

Same author

Synthesis and Characterization of Thermostable Antimicrobial Peptide-DNAzyme Conjugates for the Proof-of-Concept Detection of <i>E. coli</i>.

ACS omega·2026

Same author

Demyelination and Cognitive Performance in Long COVID Patients with Insomnia and/or Depression.

International journal of molecular sciences·2025

Same author

Mapping Charge Interactions in Intrinsically Disordered Proteins.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Same author

Assessing rapid response mobile laboratory (RRML) capacities in Europe and Africa to improve One Health disease surveillance activities and pandemic preparedness across two continents.

One health outlook·2025

Same author

Curing reaction kinetics of paper-based phenolic resin laminates-from laboratory measurements to inline quality control.

Analytical and bioanalytical chemistry·2025

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Lyophilised Reverse Transcriptase and Polymerase for Point-of-Care Diagnostics of SARS-CoV-2.

Natalie Mutter¹, Barbara Posch¹, Yasmin Gillitschka¹

¹Molecular Diagnostics, Center for Health and Bioresources, AIT Austrian Institute of Technology GmbH, Vienna, Austria.

Microbial Biotechnology

|July 24, 2025

Summary

This study developed a stable, lyophilized amplification master mix for rapid point-of-care diagnostics. The new mix successfully detected low levels of COVID-19 RNA, enabling field-based molecular pathogen detection.

Keywords:

RT‐qPCR SARS‐CoV‐2 lyophilisation point‐of‐care pulse controlled amplification recombinant enzymes

More Related Videos

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Related Experiment Videos

Last Updated: Jun 21, 2026

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Area of Science:

Biotechnology
Molecular Diagnostics
Pathogen Detection

Background:

The COVID-19 pandemic highlighted the critical need for rapid, accessible point-of-care diagnostic tests.
Current diagnostic methods often face challenges with supply chain stability and cold-chain requirements.
Developing self-sufficient reagent preparation is crucial for reliable diagnostics.

Purpose of the Study:

To create a stable, lyophilized amplification master mix for point-of-care (POC) molecular diagnostics.
To ensure independence from external suppliers and complex supply chains for diagnostic reagents.
To enable molecular diagnostics in resource-limited or field settings.

Main Methods:

Designed, expressed, and purified two off-patent amplification enzymes.
Formulated and lyophilized an amplification master mix for enhanced stability.
Integrated the lyophilized mix into a mobile, low-cost point-of-care diagnostic system.

Main Results:

The lyophilized master mix demonstrated stability for long-term storage and cold-free transport.
The integrated system successfully detected 10 copies/μL of COVID-19 RNA.
The developed system facilitates molecular diagnostics outside of traditional laboratory settings.

Conclusions:

The stable, cold-free amplification master mix is suitable for point-of-care diagnostics.
This innovation supports molecular diagnostics of pathogens in field or home settings.
The combination of stable reagents and a mobile device enhances accessibility and reduces reliance on cold chains.