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Updated: Jun 28, 2026

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications
Published on: March 8, 2024
Protocol for the establishment and characterization of South African patient-derived intestinal organoids
Naaziyah Abdulla1, Ruth Aronson2, Thea-Leonie du Plessis2
1School of Molecular and Cell Biology, Faculty of Science, University of the Witwatersrand, Private Bag 3, Johannesburg, South Africa; Infectious Diseases and Oncology Research Institute (IDORI), Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
Abstract:
Patient-derived organoids are valuable for modeling disease pathogenesis, therapeutic screening, and advancing personalized medicine. Here, we present a protocol for generating and characterizing intestinal organoids from South African patients, using both healthy and cancerous tissues. We describe steps for generating organoid cultures, quantitative reverse-transcription polymerase chain reaction (RT-qPCR), immunofluorescence microscopy, and histological staining. Notably, we provide a detailed method for cholesterol profiling in organoids and tissues, offering a powerful tool for researchers exploring lipid biology in organoid systems.
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