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Products of the Citric Acid Cycle00:53

Products of the Citric Acid Cycle

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The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
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Related Experiment Video

Updated: Sep 14, 2025

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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Graphitic nanozyme for visual ascorbic acid sensing in commercial beverages.

Xiaoyuan Sun1, Shen Wang2, Guofeng Gui1

  • 1College of Chemistry and Chemical Engineering, Guizhou University of Engineering Science, Bijie 551700, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|July 24, 2025
PubMed
Summary

A new ultra-stable nanozyme sensor offers a simple, rapid, and visual method for detecting ascorbic acid (vitamin C) in foods. This breakthrough aids in assessing vitamin C content, crucial due to its instability during processing and storage.

Keywords:
Ascorbic acidColorimetric detectionCommercial beveragesGraphitic nanozymePeroxidase–like activity

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Area of Science:

  • Nanomaterials Science
  • Analytical Chemistry
  • Food Science

Background:

  • Ascorbic acid (vitamin C) is an essential nutrient vital for human health.
  • Dietary vitamin C is susceptible to degradation during food processing and storage, necessitating accurate quantification methods.
  • Existing nanozyme sensors often lack stability in acidic conditions optimal for ascorbic acid detection.

Purpose of the Study:

  • To develop a stable and efficient nanozyme-based colorimetric sensor for quantifying ascorbic acid in food.
  • To overcome the limitations of existing sensors regarding stability and optimal reaction conditions.
  • To provide a simple, rapid, and visually detectable method for assessing vitamin C content in food products.

Main Methods:

  • Fabrication of an ultra-stable peroxidase-like Cobalt-Platinum graphitic nanozyme (CoPt@G) using chemical vapor deposition (CVD).
  • Construction of a colorimetric biosensor platform utilizing the CoPt@G nanozyme for ascorbic acid detection.
  • Validation of the sensor's performance, including linear range, detection limit, selectivity, and application in commercial beverages.

Main Results:

  • The developed CoPt@G nanozyme exhibits enhanced stability, particularly in acidic environments.
  • The colorimetric biosensor demonstrated a linear detection range of 5-60 μM for ascorbic acid.
  • A low detection limit of 1.4 μM (S/N=3) was achieved, with good selectivity and practical applicability in beverages.

Conclusions:

  • The CoPt@G nanozyme-based sensor provides a robust and sensitive platform for ascorbic acid quantification.
  • This method offers a simple, rapid, and visual approach for assessing vitamin C content in food.
  • The findings guide informed dietary choices by enabling reliable vitamin C analysis in food products.