Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Stability01:53

RNA Stability

33.9K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.9K
Riboswitches01:56

Riboswitches

8.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.5K
Types of RNA01:23

Types of RNA

64.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
64.9K
Nucleic Acid Structure01:25

Nucleic Acid Structure

7.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
7.1K
Translational Regulation01:29

Translational Regulation

98
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
98
RNA Structure01:19

RNA Structure

5.3K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

REV1 inhibition enhances trinucleotide repeat mutagenesis.

Open biology·2026
Same author

REV1 inhibition enhances trinucleotide repeat mutagenesis.

bioRxiv : the preprint server for biology·2025
Same author

Discovery of small molecules against porcine reproductive and respiratory syndrome virus replication by targeting NendoU activity.

Journal of virology·2025
Same author

Assessment of uptake of sulphadoxine-pyrimethamine for intermittent preventive treatment among pregnant women in Osun State, Nigeria.

Transactions of the Royal Society of Tropical Medicine and Hygiene·2024
Same author

Probing hot spots of protein-protein interactions mediated by the safety-belt region of REV7.

Structure (London, England : 1993)·2024
Same author

Small molecules that regulate the N<sup>6</sup>-methyladenosine RNA modification as potential anti-cancer agents.

European journal of medicinal chemistry·2024

Related Experiment Video

Updated: Sep 14, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K

Structure and function of reader proteins that recognize methylated RNA residues.

Ruth M Ogboye1, M Kyle Hadden1

  • 1Department of Pharmaceutical Sciences, University of Connecticut, 69 N Eagleville Rd, Unit 3092, Storrs, CT 06269-3092, United States.

Bioorganic Chemistry
|July 24, 2025
PubMed
Summary

RNA methylation readers are crucial proteins that recognize methylated RNA, influencing gene expression. This review details their structures, targets, and roles in human cancers, aiding drug development.

More Related Videos

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.3K
DNAzyme-dependent Analysis of rRNA 2&#8217;-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

8.4K

Related Experiment Videos

Last Updated: Sep 14, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.3K
DNAzyme-dependent Analysis of rRNA 2&#8217;-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

8.4K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Expression Regulation

Background:

  • RNA methylation is a key post-transcriptional regulatory mechanism.
  • Epigenetic 'writer', 'eraser', and 'reader' proteins mediate this reversible process.
  • RNA methylation reader proteins, which recognize methylated RNA, are less understood than general RNA-binding proteins.

Purpose of the Study:

  • To comprehensively analyze RNA methylation reader proteins.
  • To highlight their structures, targeted RNA sequences, and binding interactions.
  • To discuss their biological roles in human malignancy and therapeutic potential.

Main Methods:

  • Literature review and analysis of existing research on RNA methylation readers.
  • Structural analysis of reader proteins and their interactions with methylated RNA.
  • Examination of biological outcomes and current small molecule inhibitors.

Main Results:

  • Detailed characterization of reader protein structures, target RNA sequences, and critical binding sites.
  • Elucidation of the functional consequences of reader protein binding in human cancers.
  • Overview of the current landscape of small molecule inhibitors targeting these readers.

Conclusions:

  • RNA methylation readers play a vital role in normal and aberrant cellular signaling.
  • Understanding these readers is crucial for developing targeted drugs and probes.
  • This review provides insights for future therapeutic strategies against RNA methylation-related diseases.