Genome-wide identification, expression profiling, and functional analysis of sugar transporter family genes in Diaphorina citri
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers identified 61 sugar transporters (STs) in the Asian citrus psyllid (ACP), Diaphorina citri. Silencing specific ST genes significantly increased ACP mortality, indicating their crucial role in psyllid survival.
Area Of Science
- Entomology
- Molecular Biology
- Biochemistry
Background
- Sugar transporters (STs) are vital for cellular sugar uptake and metabolism.
- Phloem-feeding hemipterans rely on STs for growth, development, and osmoregulation.
- The specific STs in the Asian citrus psyllid (ACP), Diaphorina citri, were previously uncharacterized.
Purpose Of The Study
- To identify and characterize sugar transporters in Diaphorina citri.
- To investigate the expression patterns and functional roles of STs in ACP.
- To explore potential targets for ACP control.
Main Methods
- Genome and transcriptome analysis to identify ST genes in D. citri.
- Phylogenetic analysis and chromosome localization of ST genes.
- Quantitative real-time PCR to analyze ST gene expression in different developmental stages, tissues, and under varying dietary conditions.
- RNA interference (RNAi) to assess the function of specific ST genes.
Main Results
- A total of 61 ST genes were identified in D. citri, with most possessing 12 transmembrane regions.
- Phylogenetic analysis revealed expansions within the ST gene family.
- ST gene expression varied across developmental stages and tissues, and was influenced by diet.
- RNAi-mediated silencing of DcST16, DcST38, DcST52, and DcST56 resulted in significant adult mortality, with DcST56 knockdown causing a swollen abdomen phenotype.
Conclusions
- This study provides the first comprehensive identification and characterization of STs in Diaphorina citri.
- The identified STs play critical roles in ACP physiology, development, and survival.
- Specific ST genes represent potential targets for developing novel pest control strategies against ACP.
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