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FOXO is involved in anti-WSSV immunity by regulating autophagy-related genes
Xiaohan Liu1, Ying Huang2, Ziyue Zhu3
1State Key Laboratory of Climate System Prediction and Risk Management, Nanjing University of Information Science and Technology, Nanjing, 210044, China; School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, 210044, China; Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.
Abstract:
Forkhead box O (FOXO) interacts with autophagy related (ATG) genes to regulate autophagic activity. However, whether FOXO can regulate ATG genes to participate in crustacean infection with white spot syndrome virus (WSSV) is unknown. In this study, we identified seven ATG genes (EsGABARAP, EsBeclin-1, EsATG12, EsATG5, EsATG7, EsLC3, and EsATG9) in Eriocheir sinensis. Phylogenetic tree analysis shows that these ATG proteins of E. sinensis are evolutionarily close to other protein homologs in crustaceans. EsFOXO, as well as seven ATG genes, were widely distributed in multiple tissues, and the expression patterns indicated that their expression levels were all significantly elevated after WSSV stimulation. RNA interference analysis revealed that EsFOXO regulated the expression of ATG genes during WSSV infection and that the knockdown of EsFOXO resulted in elevated VP28 expression levels in vivo. And the survival rate of crabs infected with WSSV was significantly reduced after dsEsFOXO injection. In conclusion, FOXO may be involved in the innate immunity mechanism against WSSV in crustaceans by regulating ATG genes.
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