Related Experiment Video

Updated: Sep 14, 2025

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.8K

TRIM27 promotes ovarian cancer progression through destabilizing AMPK and thus inactivating the cGAS/STING signaling

Liyan Wang1, Huailiang Wu2, Jing Wang1

  • 1Department of Gynecology, Renmin hospital of Wuhan university, Wuhan 430060, China.

International Journal of Biological Macromolecules
|July 24, 2025
PubMed

Insights

Tripartite motif-containing 27 (TRIM27) drives ovarian cancer (OV) by suppressing immune surveillance. Inhibiting TRIM27 reduces cancer growth and may offer a new therapeutic target for this malignancy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Ovarian cancer (OV) is a deadly gynecologic malignancy with poor prognosis due to late diagnosis and treatment resistance.
  • Tripartite motif-containing 27 (TRIM27), a regulator in cancers, has an underexplored role in OV.
  • Understanding TRIM27's function is crucial for developing novel OV therapies.

Purpose of the Study:

  • To investigate the role and mechanism of TRIM27 in ovarian cancer.
  • To determine if TRIM27 expression correlates with clinical outcomes in OV patients.
  • To explore TRIM27 as a potential prognostic biomarker and therapeutic target for OV.

Main Methods:

  • Analysis of TRIM27 expression in OV tissues and cell lines.
  • Gain- and loss-of-function assays to assess TRIM27's impact on OV cell behavior.
  • RNA sequencing, immunoprecipitation, mass spectrometry, and in vivo ubiquitination assays to elucidate molecular mechanisms.
  • Correlation analysis between TRIM27 expression and clinical outcomes.

Main Results:

  • TRIM27 is significantly upregulated in ovarian cancer tissues and cell lines.
  • TRIM27 depletion inhibits OV cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition (EMT).
  • TRIM27 acts as a ubiquitin ligase, degrading AMPKα via K48-linked ubiquitination, suppressing the cGAS-STING signaling pathway and immune surveillance.
  • Elevated TRIM27 expression is linked to poor clinical outcomes in OV patients.

Conclusions:

  • TRIM27 is an oncogenic driver in ovarian cancer, promoting tumorigenesis by evading immune surveillance.
  • TRIM27 functions by mediating AMPKα degradation and suppressing the cGAS-STING pathway.
  • TRIM27 represents a promising prognostic biomarker and therapeutic target for ovarian cancer.
Keywords:
Ovarian cancerTRIM27UbiquitinationcGAS-STING

More Related Videos

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.8K
Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence08:55

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Published on: November 2, 2014

12.4K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.9K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.5K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
4.0K