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miR-126-3p Protects Neonatal Rats With Hypoxic-Ischemic Brain Damage Through Targeting LPR6 via PKC/ERK Signaling

W Ma1, H Li, L Zha

  • 1Pediatric Department, Puren Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, China. m54malaoshi@hotmail.com.

Physiological Research
|July 25, 2025
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Summary

MicroRNA-126-3p (miR-126-3p) protects against neonatal hypoxic-ischemic brain damage (HIBD) by downregulating LRP6 and activating the PKC/ERK pathway, reducing inflammation and improving neurological outcomes.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Neonatal hypoxic-ischemic brain damage (HIBD) is a significant cause of neonatal mortality.
  • The role of miR-126-3p in HIBD pathogenesis is not fully understood, despite its decreased levels in cerebral hemorrhage patients.

Purpose of the Study:

  • To elucidate the mechanism of miR-126-3p in neonatal hypoxic-ischemic brain damage (HIBD).
  • To investigate the regulatory relationship between miR-126-3p and low-density lipoprotein receptor-related protein 6 (LRP6).

Main Methods:

  • Established a neonatal rat model of HIBD using the Rice-Vannucci method.
  • Utilized bioinformatics databases and dual-luciferase assays to identify and validate miR-126-3p targets.
  • Administered miR-126-3p and LRP6 manipulations, and assessed pathological changes, neurological function, and molecular signaling pathways (PKC/ERK).

Main Results:

  • miR-126-3p knockdown exacerbated HIBD, while its overexpression ameliorated inflammation, brain pathology, and neurological deficits.
  • miR-126-3p was confirmed to directly target and downregulate LRP6 expression.
  • Overexpression of miR-126-3p improved HIBD outcomes by downregulating LRP6 and activating the protein kinase C (PKC)/extracellular signal-regulated kinase (ERK) pathway.

Conclusions:

  • miR-126-3p plays a protective role in HIBD.
  • The protective effect is mediated through the miR-126-3p/LRP6 axis and subsequent activation of the PKC/ERK signaling pathway.
  • Targeting miR-126-3p represents a potential therapeutic strategy for neonatal hypoxic-ischemic brain damage.